Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mouse tracheal pseudostratified vs hillock basal stem cells

ABSTRACT: Mouse tracheal pseudostratified vs hillock basal stem cells

PROVIDER: PRJNA1063662 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

ATACseq comparison of mouse tracheal pseudostratified vs hillock basal stem cells

Project description:ATACseq comparison of mouse tracheal pseudostratified vs hillock basal stem cells

| PRJNA1063667 | ENA

RNAseq transcriptomic comparison of mouse tracheal pseudostratified vs hillock basal stem cells

Project description:RNAseq transcriptomic comparison of mouse tracheal pseudostratified vs hillock basal stem cells

| PRJNA1063665 | ENA

ATACseq comparison of mouse tracheal pseudostratified vs hillock basal stem cells

Project description:Airway hillocks are stratified epithelial structures of unknown function. We show that the luminal cells of hillocks are squamous barrier cells that shield a unique population of underlying hillock basal stem cells. Hillocks occur in characteristic locations and persist for months in uninjured mice. Following injury, hillock basal stem cells undergo massive clonal expansion, resurface denuded airway, and demonstrate extreme plasticity, generating classic airway epithelium with its six component cell types. Remarkably, hillocks resist a very broad spectrum of injuries, including toxins, infection, acid, and physical injury. Furthermore, hillock basal stem cells are characterized by enhanced expression associated with barrier function, cell adhesion, and retinoic acid catabolism. Indeed, hillock basal cells preferentially expand, stratify, and keratinize in the setting of retinoic acid signaling inhibition, a known clinical cause of squamous metaplasia. Finally, we identify human hillocks whose basal cells demonstrate preferential sensitivity to retinoic acid inhibition and conserved barrier properties. Given their capacity for clonal expansion, their plasticity, and their retinoic acid sensitivity, the existence of hillocks and hillock basal stem cells have broad implications for injury repair and for defining the origin of squamous metaplasia, long thought to be a precursor of lung cancer.

2024-03-05 | GSE253020 | GEO

RNAseq transcriptomic comparison of mouse tracheal pseudostratified vs hillock basal stem cells

2024-03-05 | GSE253021 | GEO

mTOR Inhibition Enables Long-Term Expansion of Human Neonatal Tracheal Aspirate-Derived Airway Basal Stem Cells

Project description:Bronchopulmonary dysplasia remains one of the most common complication of prematurity, despite significant improvements in perinatal care. Functional modeling of human lung development and disease, like BPD, is limited by our ability to access the lung and to maintain relevant stem cell populations in culture. Single cell RNA-sequencing confirmed the presence of epithelial cells in tracheal aspirates obtained from intubated neonates. Using combined SMAD signaling inhibition and mTOR inhibition neonatal tracheal-aspirate derived (nTAD) basal stem cells can be expanded long-term and retain the ability to differentiate into pseudo-stratified airway epithelium. Conclusions: Our data demonstrate that neonatal tracheal aspirate-derived epithelial cells can provide a novel ex vivo human cellular model to study neonatal lung development and disease.

2020-09-21 | GSE137950 | GEO

mTOR Inhibition Enables Long-Term Expansion of Human Neonatal Tracheal Aspirate-Derived Airway Basal Stem Cells

2020-04-15 | GSE137353 | GEO

Gene expression in mouse tracheal basal cells

2009-07-02 | GSE15724 | GEO

Transcription profiling of mouse tracheal basal cells

Project description:Epithelial basal cells (BCs) are an important stem cell population of the airways. We purified BCs from a KRT5-GFP transgenic mouse line and used Affymetrix arrays to compare there gene expression to that of non-BC epithelium. Experiment Overall Design: Three populations of cells were obtained by FACS: 1. KRT5-GFP-, lectin- columnar epithelium. 2. KRT5-GFP-, lectin+ BCs. 3. KRT5-GFP+, lectin+ BCs. Three biological replicates of each sample were hybridized to Affymetrix microarray.

2009-07-11 | E-GEOD-15724 | biostudies-arrayexpress

Identification of early targets of SOX2 and PI3K signaling in normal, patient-derived tracheal basal cell lines.

Project description:Microarrays were used to examine gene expression changes in patient derived tracheal basal cell lines after treatment with Lenti-SOX2. Comparisons were made between baseline, Lenti-SOX2 and Lenti-SOX2 with a PI3K inhibitor (BKM120).

2015-07-01 | GSE59866 | GEO

Mouse tracheal pseudostratified vs hillock basal stem cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2024-03-05 | GSE253023 | GEO