Project description:MicroRNAs (miRNAs) are endogenous small noncoding RNAs (18–25 nt) that are involved in many physiological processes including development, cancer, immunity, apoptosis and host-microbe interactions through posttranscriptional regulation of gene expression. In this study, we measured the profile of small RNAs over the developmental transitions of Spodoptera frugiperda from egg to adult. We identified 741 miRNAs, including 493 known and 248 novel miRNAs. In addition, we identified the miRNAs differentially expressed over the developmental transitions via weighted gene co-expression network analysis (WGCNA). This study identified critical miRNAs involved in the transitions of this important pest insect S. frugiperda from egg to adult, thus providing a useful resource for exploring the regulatory role of miRNAs during insect post-embryonic development.
Project description:This SuperSeries is composed of the following subset Series: GSE16775: Effect of HdIV or MdBV injection on the Spodoptera frugiperda hemocyte transcriptome GSE16776: Effect of HdIV or MdBV injection on the Spodoptera frugiperda fat body transcriptome Refer to individual Series
Project description:Purpose: We analyzed the 3rd-instar Spodoptera frugiperda response after SfAV-1a infection. Specifically, we targeted three gene types in the infected host namely, mitochondrial, cytoskeleton and innate immunity genes.
Project description:We report the application of whole transcriptome sequencing technology for high-throughput profiling of coding and non-coding RNAs associated with Spodoptera frugiperda feeding in Zea mays. 4,366 mRNAs and 233 lncRNAs were differentially expressed during Spodoptera frugiperda feeding in Zea mays. Our data contribute to the understanding of the function of coding and non-coding RNAs in the regulation of plant-insect interactions.
Project description: In order to clarify the molecular mechanism of metabolic detoxification in Spodoptera frugiperda, the enzyme activity assay and transcriptome sequencing analysis were used to screen and identify the relevant genes related to metabolic detoxification after induced by insecticides at LC50 dose.
Project description:Comparison of the effects of 2 polydnaviruses (HdIV vs MdBV) injection on L5 Spodoptera frugiperda larvae hemocytes transcriptome. The experimental design is as follows: 3 biological replicates with dye swap. Each treatment (HdIV or MdBv) is compared to control treatment (PBS).
