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ABSTRACT: Effects of plum tree intercropping on the growth and rhizosphere soil microorganisms of Polygonatum cyrtonema Hua. based on metagenomics

PROVIDER: PRJNA1064224 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR27532987_1.fastq.gz	Fastqsanger.gz
	SRR27532987_2.fastq.gz	Fastqsanger.gz
	SRR27532988_1.fastq.gz	Fastqsanger.gz
	SRR27532988_2.fastq.gz	Fastqsanger.gz
	SRR27532989_1.fastq.gz	Fastqsanger.gz

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Project description:Intercropping is a vital technology in resource-limited agricultural systems with low inputs. Peanut/maize intercropping enhances iron (Fe) nutrition in calcareous soil. Proteomic studies of the differences in peanut leaves, maize leaves and maize roots between intercropping and monocropping systems indicated that peanut/maize intercropping not only improves Fe availability in the rhizosphere but also influences the levels of proteins related to carbon and nitrogen metabolism. Moreover, intercropping may enhance stress resistance in the peanut plant (Xiong et al. 2013b). Although the mechanism and molecular ecological significance of peanut/maize intercropping have been investigated, little is known about the genes and/or gene products in peanut and maize roots that mediate the benefits of intercropping. In the present study, we investigated the transcriptomes of maize roots grown in intercropping and monocropping systems by microarray analysis. The results enabled exploration differentially expressed genes in intercropped maize. Peanut (Arachis hypogaea L. cv. Luhua14) and maize (Zea mays L. cv. Nongda108) seeds were grown in calcareous sandy soil in a greenhouse. The soil was enhanced with basal fertilizers [composition (mg·kg−1 soil): N, 100 (Ca (NO3)2·4H2O); P, 150 (KH2PO4); K, 100 (KCl); Mg, 50 (MgSO4·7H2O); Cu, 5 (CuSO4·5H2O); and Zn, 5 (ZnSO4·7H2O)]. The experiment consisted of three cropping treatments: peanut monocropping, maize monocropping and intercropping of peanut and maize. After germination of peanut for 10 days, maize was sown. Maize samples were harvested after 63 days of growth of peanut plants based on the degree of Fe chlorosis in the leaves of monocropped peanut. The leaves of monocropped peanut plants exhibited symptoms of Fe-deficiency chlorosis at 63 days, while the leaves of peanut plants intercropped with maize maintained a green color.

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Comparative Proteome Profiles of Polygonatum cyrtonema Hua Rhizomes (Rhizoma Ploygonati) in Response to Different Levels of Cadmium Stress

Project description:The Polygonatum cyrtonema Hua rhizomes (Rhizoma Polygonati, RP) are consumed for health benefits. The main source of the RP is wild P. cyrtonema populations in the Hunan province of China. However, the soil Cadmium (Cd) content in Huanan is increasing, thus increasing the risks of Cd accumulation in RP and ending up in the human food chain. We subjected different levels of Cd stress to study the protein regulation mechanism under cadmium stress of P. cyrtonema.

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