ABSTRACT: Effects of Lycium barbarum branches and leaves on growth performance, slaughter performance, rumen fermentation parameters and rumen microbiota in Hu sheep
Project description:Lycium barbarum residue contains abundant bioactive nutrients which can be used as feed supplement. This study investigated the effects of fermented and non-fermented Lycium barbarum residues (RFW and RW) on the meat quality and immunity of sheep (Ovis aries). Fifty-four Tan sheep were randomly divided into control, RFW or RW treatments. Data showed that RFW and RW increased the carcass weight, fat content, ash content and reduced the cooking loss of lamb. RFW performed more significant effects on activating immune-related genes than those of RW. The expression of chemokines and immune-related pathways, such as signaling pathways of interleukin-17 signaling pathway and NOD-like receptor signaling pathway, were elevated in sheep fed RFW. RW increased the diversity in rumen metabolites, especially compositions of lipids, organic acids and organ heterocyclic compounds. RFW affected numerous compounds which are closely correlated with the activation of immune genes. In conclusion, RFW could represent a valuable strategy to improve growth performance and immunity of sheep
Project description:To investigate the protective effect of Lycium barbarum polysaccharide-glycoprotein (LBP) in the radiation-induced HaCaT cell injury, We performed gene expression profiling analysis using data obtained from RNA-seq of HaCaT cells at 3h after radiation.
2024-06-01 | GSE218507 | GEO
Project description:Lycium barbarum byproducts improve the rumen microbial community structure and promote rumen fermentation in sheep grazing on sown pastures
Project description:Ruminant livestock are one of the major contributors to carbon emission contributing the global warming issue. Methane (CH4) produced from enteric microbial fermentation of feed in the reticulo-rumen are known to differ between sheep with different digestive function and fermentation products such as metabolites. However, the molecular mechanism underpinning differences in methane emission remains to be fully elucidated. We extracted a membrane and cytosolic protein fraction of rumen epithelium proteins from both high (H) and low (L) CH4 emitting sheep. Protein abundance differences between the phenotypes were quantified using SWATH-mass spectrometry. We identified 92 proteins annotated as cell surface transporters, of which only solute carrier family (SLC) 40A1 had a greater fold change of protein expression in the high methane emission phenotype. The main difference in protein abundance we found were related to the metabolism of glucose, lactate and processes of cell defence against microbes in the epithelium of sheep in each group. To best of our knowledge, this represents one of the most comprehensive proteomes of ovine rumen epithelium to date.
