Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare transcriptome profiling (RNA-seq) between HaCaT cell and OSM-treated HaCaT cells Methods: mRNA profiles of HaCaT cells and OSM-treated HaCaT cells were generated by deep sequencing, in triplicate, using Illumina GAIIx. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks.
Project description:Transcriptome profiling by using RNA-seq was performed on bleogen pB1-treated, EGF-treated, and untreated HaCaT keratinocytes to gain further insights into the wound healing effects of bleogen pB1 and EGF
Project description:Transcriptional profiling in HACAT cells using a whole human genome array; HACAT cells treated with si RNA against Keap 1 or a scrambled si RNA sequence (Scram) vs HACAT cells mock transfected with lipofectamine (reference control) Experiment Overall Design: 2 biological replicates, 2 technical (dye swap) replicates per treatment.
Project description:Obacunone treatment upregulates gene expression of cholesterol metabolism-associated genes in HaCaT cells Total RNA obtained from HaCaT cells treated with 100 uM obacunone for 0.5, 1, 3, 6, or 12 h was compared to that of vehicle control cells.
Project description:Obacunone treatment upregulates gene expression of cholesterol and lipid metabolism-associated genes in HaCaT cells Total RNA obtained from HaCaT cells treated with 10, 50, or 100 uM obacunoe for 4 h was compared to that of vehicle control cells.
Project description:This RNA sequencing dataset analyzes circRNA expression changes in HaCaT keratinocytes after UVA irradiation. Cells were exposed to 5 J/cm2 UVA and profiled using ribosomal RNA-depleted RNA sequencing on an Illumina platform. Differentially expressed circRNAs were identified by computational pipelines. The data provide insights into circRNA regulation during photo-oxidative stress.