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Chemotactic Signalling Triggers Dental Pulp Fibrosis in the Stem Cell Niches During

ABSTRACT: Chemotactic Signalling Triggers Dental Pulp Fibrosis in the Stem Cell Niches During

PROVIDER: PRJNA1064909 | ENA |

REPOSITORIES: ENA

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Chemotactic Signalling Triggers Dental Pulp Fibrosis in the Stem Cell Niches During

Project description:Aging often triggers dental pulp fibrosis, leading to various clinical repercussions, including heightened vulnerability to dental infections, compromised tooth vitality, and reduced responsiveness to dental interventions. Despite its common occurrence in clinical settings, the precise molecular mechanisms driving this condition remain elusive. Leveraging single-cell transcriptome analysis from both our own and publicly available datasets, we identified Ccrl2+ macrophages as particularly prone to susceptibility during the early stages of aging. Notably, dental pulp progenitors exhibiting high expression of Rarres2, a unique ligand for Ccrl2, orchestrate the selective recruitment of a specific macrophage population to the stem cell niches. Subsequently, this results in the presentation of the ligand-receptor complex to Cmklr1, a receptor ubiquitously expressed among all macrophage populations, ultimately leading to macrophage activation and expansion via the Rarres2/Ccrl2/Cmklr1 axis. Our study, supported by rigorous experimental validation, conclusively shows that macrophage activation and expansion in stem cell niches lead to increased secretion of proinflammatory factors, which contribute to dental pulp fibrosis during aging. Our data reveals the complex molecular dynamics of dental pulp aging, particularly focusing on interactions within the immune microenvironment. It offers a novel perspective on potential treatments for age-related pulp diseases, highlighting the role of macrophages and the possibility of modifying the immune microenvironment for therapeutic benefit.

2025-01-29 | GSE253259 | GEO

Dental pulp microbiome

Project description:Dental pulp

| PRJEB50897 | ENA

Gene expression analysis of human permanent dental pulp

Project description:Gene expression analysis data of human permanent dental pulp tissues

2016-11-22 | GSE75644 | GEO

Insulin-like Growth Factor Binding Protein 7 identified in aged dental pulp by single-cell RNA sequencing

Project description:Dental pulp plays a crucial role for dental health, and dental pulp aging influences their regenerative and reparative function. However, the underlying molecular mechanisms of dental pulp aging are not exhaustively understood, and thereby an in-depth and complete understanding of the aged dental pulp is of foremost importance. This study aimed to explore the heterogeneity of young and aged dental pulp tissue using single-cell RNA sequencing (scRNA-seq).

2024-12-15 | GSE274562 | GEO

Characterization of human dental pulp cells grown in chemically defined serum-free medium

Project description:Dental pulp cells obtained from several donors proliferated actively in a serum-free medium STK2. The growth rate of dental pulp cells from most donors was higher in the serum-free medium than that in a medium containing 10% serum. DNA microarray analyses showed that gene expression profile of dental pulp cells grown in the serum-free medium was similar to that of cells grown in a medium containing 10% serum. However, several genes related to cell proliferation were up-regulated in dental pulp cells grown in the serum-free medium.

2018-03-14 | GSE97199 | GEO

SAHA Rat Dental Pulp

Project description:Transcriptional response of rat dental pulp cells (DPCs) cultured with SAHA at early and late mineralisation time points Transcript profiling of DPC identified several novel genes expression induced and supressed by HDACi at 24 hrs and 14 days under mineralising conditions. SAHA induces several members of the MMP family of endopepsidases (TIMP-1, MMP-9, MMP-13) and other members of the endochondral ossification pathway at 24 h. 8 experiemental parameters were analysed, each carried out in quadruplicate

2015-06-16 | E-GEOD-67175 | biostudies-arrayexpress

Comparative gene expression analysis of the human dental pulp in deciduous and permanent teeth

Project description:Human deciduous and permanent teeth exhibit different developmental processes, morphologies, histological characteristics and life cycles. In addition their pulp tissues react differently to external stimuli, such as the pulp sensitivity test, dental trauma and pulp therapy materials. These differences are attributable to their genetic backgrounds. Therefore the purpose of this study is to compare the differences of dental pulp in deciduous and permanent teeth. Pulp samples were obtained from permanent premolars (n=6, aged 11-14 years) and deciduous teeth (n=6, aged 11-14 years). Comparative cDNA microarrary analysis revealed several differences in gene expression between the deciduous and permanent pulp tissues. Each GSM record represents a pulp sample pooled from two teeth samples.

2014-08-21 | E-GEOD-51449 | biostudies-arrayexpress

Effect of EtOH on transcriptome signatures in human dental pulp stem cells

Project description:We have performed gene expression microarray analysis to profile transcriptomic signatures affected by EtOH in human dental pulp stem cells Established human dental pulp stem cells were treated with different dose of EtOH (0, 1, 5, 10, 20 and 50mM) for a different time periods (24 and 48 hrs). Total RNA was extracted and subjected to gene expression microarray analysis using Affymetrix human genome 2.0 plus array

2014-05-03 | E-GEOD-57255 | biostudies-arrayexpress

Differential gene expression of Wnt ligands/Wnt agonist treated human dental pulp stem cells

Project description:Wnt regulates various cell responses. In dental pulp cells, Wnt signaling control cell proliferation, apoptosis, migration and differentiation. Here, the differential gene expression of human dental pulp stem cells treated with Wnt ligands or Wnt agonist was examined using a high throughput RNA sequencing technique. Results demonstrated that Wnt ligands or Wnt agonist altered numerous gene expression in human dental pulp stem cells.

2022-12-09 | GSE183444 | GEO

RNA-Seq analysis of neurons derived from induced pluripotent stem cells reprogrammed from dental pulp

Project description:In this study, we investigated its suitability for disease modeling by carrying out gene expression profiling, using RNA-seq, on neurons derived from iPSCs made from dental pulp extracted from deciduous teeth (T-iPSCs) and fibroblasts (F-iPSCs). Comparison of expression profiles of iPSC derived from dental pulp and skin-fibroblast

2013-05-01 | E-GEOD-43143 | biostudies-arrayexpress

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