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Humanizing the RNA regulator NOVA1 in mice alters their vocal communication

ABSTRACT: Humanizing the RNA regulator NOVA1 in mice alters their vocal communication

PROVIDER: PRJNA1064990 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR27554605_1.fastq.gz	Fastqsanger.gz
	SRR27554605_2.fastq.gz	Fastqsanger.gz
	SRR27554606_1.fastq.gz	Fastqsanger.gz
	SRR27554606_2.fastq.gz	Fastqsanger.gz
	SRR27554607_1.fastq.gz	Fastqsanger.gz

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Humanizing the RNA regulator NOVA1 in mice alters their vocal communication

Project description:This SuperSeries is composed of the SubSeries listed below.

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Nova1 is a master regulator of alternative splicing in pancreatic beta cells

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Transcription in wounded rat vocal folds and vocal fold fibroblasts

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2014-10-09 | E-GEOD-62204 | biostudies-arrayexpress

Nova1 is a master regulator of alternative splicing in pancreatic beta cells

Project description:Alternative splicing (AS) is a fundamental mechanism for the regulation of gene expression. It affects more than 90% of human genes but its role in the regulation of pancreatic beta cells, the producers of insulin, remains unknown. Our recently published data indicated that the “neuron specific” Nova1 splicing factor is expressed in pancreatic beta cells. We have presently coupled specific knockdown (KD) of Nova1 with RNA-sequencing to determine all splice variants and downstream pathways regulated by this protein in beta cells. Nova1 KD altered the splicing of nearly 5000 transcripts. Pathway analysis indicated that these genes are involved in exocytosis, apoptosis, insulin receptor signalling, splicing and transcription. In line with these findings, Nova1 silencing inhibited insulin secretion and induced apoptosis basally and after cytokine treatment in rodent and human beta cells. These observations identify a novel layer of regulation of beta cell function, namely AS controlled by key splicing regulators such as Nova1.

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Transcription in wounded rat vocal folds and vocal fold fibroblasts

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Project description:To elucidate the function of Nova1 in neurons, especially in inhibitory neurons, gene expression profiling analysis (RNAseq, TRAP-seq) was performed using Nova1 conditional knockout mice and their primary neurons.

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Expression data from rat vocal fold at different periods after vocal fold injury

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Down-regulation of Barley Regulator of Telomere Elongation Helicase 1 (RTEL1) Alters the Distribution of Meiotic Crossovers

Project description:Down-regulation of Barley Regulator of Telomere Elongation Helicase 1 (RTEL1) Alters the Distribution of Meiotic Crossovers

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