Project description:The mature leaves (full-expanded leaves) and developing leaves (30%length of full-expanded) of 8 species of the genus Flaveria (F.pringlei, F.robusta, F.floridana, F.ramosissima, F.brownii, F.palmeri, F.bidentis and F.trinervia) Transcriptome analysis. Plants were grown in the growth chamber (12hr light - 12hr dark, 24℃, 200-300umol m-2 s-1). Total RNA was isolated from the youngest full expanded leaves or developing leaves (30%length of full-expanded) of each species.
Project description:Here we describe a method to target, multiplex and sequence full-length, native single-molecule the human mitochondrial genome utilizing the RNA-guided DNA endonuclease Cas9.
