Project description:To better understand the molecular mechanisms of the response of arbuscular mycorrhizal S. cannabina to salt stress, the transcriptional profile in both mycorrhizal and non-mycorrhizal Sesbania cannabina subjected to 3 and 27 hours NaCl treatment respectively were performed using the Illumina HiSeq™ 2000 sequencing platform (Illumina Inc., San Diego, CA, USA). Bioinformatic analysis of transcriptome data was performed to allow identification and functional annotation of differentially expressed genes (DEGs). By comparing the different genes appeared in control and treatment groups, we can learn more about the species-specific responses employed by S. cannabina and find novel associated genes or strategies in mycorrhizal plant under salt stress.
Project description:Earlier, we reported that root nodulation was inhibited by blue light irradiation of Lotus japonicus. Because some legumes do not establish nodules exclusively on underground roots, we investigated whether nodule formation in Sesbania rostrata, which forms both root and "stem" nodules following inoculation with Azorhizobium caulinodans, is inhibited by blue light as are L. japonicus nodules. We found that neither S. rostrata nodulation nor nitrogen fixation was inhibited by blue light exposure. Moreover, although A. caulinodans proliferation was not affected by blue light irradiation, bacterial survival was decreased. Therefore, blue light appears to impose different responses depending on the legume-rhizobial symbiosis.
Project description:Sesbania cannabina is an annual plant widely distributed in south China, southeast Asia, and Australia. In the present study, we sequenced the complete chloroplast genome of S. cannabina, which is 153,978 bp in length and it is characterized by a typical quadripartite structure composed of a LSC (85,406 bp) and SSC (19,112 bp) region interspersed by a pair of 23,730 bp IR regions. It encodes 125 genes, comprising 81 protein-coding genes, 8 rRNA genes, and 36 tRNA genes. Phylogenetic analysis confirmed the position of S. cannabina within the subfamily Papilionoideae.
| S-EPMC7706799 | biostudies-literature
Project description:RNA sequencing of Sesbania rostrata