Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1067103 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Ghd7 regulates a range of functions in growth and development in response to environmental cues to maximize the reproductive success of the rice plant. We used microarrays to detail the regulated genes in OX-Ghd7HJ19 transgenic plants. To gain clues to downstream genes regulated by Ghd7, we performed a microarray analysis using Affimetrix rice gene chips. Young leaves in vegetative stage (35-days old) and developing panicles (0.1cm) from field-grown OX-Ghd7HJ19 transgenic and wild-type plants with two biological replicates were used to isolate RNA for chip analysis.
2014-09-01 | E-GEOD-51616 | biostudies-arrayexpress
Project description:Analysis of leaves from 3-week old transgenic plants overexpressing AAF, which is a senescence-associated gene. Overexpression of AAF promoted leaf senescence and accumulation of reactive oxygen species (ROS) in transgenic plants. Results provide insight in the role of AAF in ROS homeostasis and the function in leaf senescence. 3-week-old plants of Col-0 (wild type, as control) and AAF-OX (overexpression line) were grown in long day condition (16hr light/8hr dark). Rosette leaves of Col-0 and AAF-OX were used for total RNA extraction and cDNA synthesis. cDNA labeling, array hybridization, and scanning followed standard Affymetrix expression array protocol. Two independent sets of microarray analyses were performed in this study.
2010-09-30 | E-GEOD-18336 | biostudies-arrayexpress
Project description:OsRUS1-GFP overexpression (OsRUS1-OX) transgenic rice lines were generated using ZH-11 wildtype. Under well-watered conditions, the leaves of OsRUS1-OX transgenic rice lines could roll in about four minutes under sunlight, and the rolled leaves of OsRUS1-OX transgenic rice lines could expand in about seven minutes if the sunlight was shaded, while the leaves of wildtype ZH-11 expanded all the times at the same conditions. The mechanism behind the light-responded rapid and dynamic leaf rolling phenotype of OsRUS1-OX transgenic rice lines is unknown. Therefore, in order to understand this mechanism the RNA-Seq approach was used to explore the expressed genes difference between OsRUS1-OX and ZH-11.
2019-03-27 | GSE128886 | GEO
Project description:Over expression of a transcription factor OsEREBP1 results in attenuation of disease symptoms upon infection with bacterial pathogen Xanthomonas oryzae pv. oryzae and tolerance to drought stress in transgenic rice plants. Microarray analysis was performed to identify genes regulated by the rice transcription factor OsERBP1. Four independent replicates of the experimental OsEREBP1-ox transgenic plants and the control non-transgenic Kitaake plants were grown under normal conditions.
2013-11-15 | E-GEOD-46173 | biostudies-arrayexpress
Project description:RNA-Seq analysis of IbBBX24 transgenic sweet potato plants
| PRJNA588771 | ENA
Project description:Purpose: we performed comparative RNA-seq analyses to identify differentially expressed genes between CsDML OX transgenic lines Wild Type Methods: Expression profiles were examined by RNA sequencing (RNA-Seq) in SD-induced (for 28 days) shoot apices of CsDML over-expressor and wild-type hybrid poplars. For RNA extraction, 25 apices from 25 trees of each WT, CsDML OX1 and CsDML OX3 genotypes were dissected under a binocular loupe to remove leaflets surrounding the shoot apical meristem Results: Using an optimized data analysis workflow, 318 differentially expressed genes (DEG) were identified in CsDML OX, among which 79 were found upregulated and 239 downregulated
2017-09-23 | GSE87155 | GEO