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The emergence of the stem cell-associated Sox and POU transcription factors predates animal origins

ABSTRACT: The emergence of the stem cell-associated Sox and POU transcription factors predates animal origins

PROVIDER: PRJNA1067618 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR27682408_1.fastq.gz	Fastqsanger.gz
	SRR27682408_2.fastq.gz	Fastqsanger.gz
	SRR27682409_1.fastq.gz	Fastqsanger.gz
	SRR27682409_2.fastq.gz	Fastqsanger.gz
	SRR27682410_1.fastq.gz	Fastqsanger.gz

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The emergence of the stem cell-associated Sox and POU transcription factors predates animal origins (SELEX)

Project description:Pluripotent stem cells are a hallmark of animal multicellularity. Sox and POU family transcription factors are pivotal for stemness and were believed to be animal innovations as they were reported absent from the genomes of their unicellular relatives. Here we describe new unicellular holozoan orthologues to Sox and POU families, indicating that they emerged before the appearance of animals. We show that choanoflagellate and filasterean Sox genes have DNA binding specificity similar to Sox2. Choanoflagellate Sox can partner with the POU member Oct4 on DNA elements found in pluripotency enhancers. Consistently, choanoflagellate – but not filasterean – Sox genes can replace Sox2 to reprogram mouse somatic cells into induced pluripotent stem cells (iPSC). In contrast, choanoflagellate POU harbors a unique DNA-binding profile that differs from Oct4 and cannot generate iPSCs. Pluripotency reprogramming with reconstructed ancestral Sox genes shows that their molecular ability to induce stemness was already present in the last common ancestor of animals and their unicellular relatives. Our findings imply that the evolution of stem cells exploited a pre-existing set of transcription factors, where the critical innovation involved an initial change in DNA specificity of POU and the exaptation of the ancestral capacity to interact with Sox transcription factors.

2024-10-23 | GSE253887 | GEO

The emergence of the stem cell-associated Sox and POU transcription factors predates animal origins (Spec-Seq)

2024-10-23 | GSE253886 | GEO

The emergence of the stem cell-associated Sox and POU transcription factors predates animal origins (SELEX)

Project description:The emergence of the stem cell-associated Sox and POU transcription factors predates animal origins (SELEX)

| PRJNA1067620 | ENA

The emergence of the stem cell-associated Sox and POU transcription factors predates animal origins (Spec-Seq)

Project description:The emergence of the stem cell-associated Sox and POU transcription factors predates animal origins (Spec-Seq)

| PRJNA1067621 | ENA

The emergence of the stem cell-associated Sox and POU transcription factors predates animal origins

Project description:This SuperSeries is composed of the SubSeries listed below.

2024-10-23 | GSE253888 | GEO

Selective influence of Sox2 on POU transcription factor binding in embryonic and neural stem cells

Project description:Embryonic stem cell (ESCs) identity is orchestrated by co-operativity between the transcription factors (TFs) Sox2 and the class V POU-TF, Oct4 at composite Sox/Oct motifs. Neural stem cells (NSCs) lack Oct4 but express Sox2 and class III POU-TFs. This raises the question of how Sox2 interacts with POU-TFs to transcriptionally specify ESCs or NSCs. Here we show that Oct4 alone binds the Sox/Oct motif and the octamer-containing palindromic MORE equally well. Sox2 binding selectively increases the affinity of Oct4 for the Sox/Oct motif. In contrast, Oct6 binds preferentially to the MORE, and is unaffected by Sox2. ChIP-seq in NSCs shows the MORE to be the most enriched motif for class III POU-TFs, with MORE sub-types apparent, but no Sox/Oct motif enrichment. These results suggest that in NSCs, co-operativity between Sox2 and class III POU-TFs may not occur and that POU-TF driven transcription uses predominantly the MORE cis architecture. Thus, distinct interactions between Sox2 and POU-TF subclasses distinguish pluripotent ESCs from multipotent NSCs, providing molecular insight into how Oct4 alone can convert NSCs to pluripotency.

2015-08-21 | GSE69859 | GEO

Panarthropod Sox genes

Project description:Embryonic expression patterns of panarthropod Sox genes

| PRJEB22589 | ENA

The hypothalamus predates the origin of vertebrates

Project description:The hypothalamus predates the origin of vertebrates

| PRJNA699670 | ENA

Sequentially acting Sox transcription factors in neural lineage development

Project description:This SuperSeries is composed of the following subset Series: GSE33059: Sequentially acting Sox transcription factors in neural lineage development [ChIP-seq] GSE33060: Sequentially acting Sox transcription factors in neural lineage development [RNA-seq] GSE33061: Sequentially acting Sox transcription factors in neural lineage development [microarray] Refer to individual Series

2011-11-01 | E-GEOD-33024 | biostudies-arrayexpress

Converging Effects of Three Different Endocrine Disrupters on Sox and Pou Gene Expression in Developing Rat Hippocampus: Possible Role of microRNA in Sex Differences

Project description:Abstract. Transcriptomes of developing, early postnatal rat hippocampus were compared after gestational and lactational exposure to different environmental chemicals exhibiting endocrine activity (endocrine disrupters) at doses impairing memory in adult offspring: Aroclor 1254 (PCB mixture) and bisphenol A, 5 or 0.5 mg/kg administered in chow, and chlorpyrifos, 3 or 1 mg/kg injected subcutaneously. All three chemicals exhibited a common effect on Sox6, Sox11, Pou2f2/Oct2, and Pou3f2/Brn2 in male hippocampus at the higher dose level. Additional Sox and Pou genes were affected by two treatments. Real time RT PCR in samples not used for transcriptomics confirmed the transcriptomics data and showed a comparable expression change for bisphenol A also at the lower dose. No changes were seen in female hippocampus. In the case of Sox6, gene network analyses indicated that miR-24 may be involved in the sexual dimorphism. This miR, which represses Sox6 and is repressed by estrogen receptor-alpa in other tissues, was downregulated by all chemicals in male but not female hippocampus. Sox6 expression levels of individual hippocampus samples (FPKM) were inversely correlated with expression levels of Pvalb, a downstream target of Sox6 and marker of an interneuron subpopulation, but not with markers of Sox6-independent interneuron subpopulations, Nos1 and 5HT3aR, indicating a selective effect on a hippocampal interneuron subpopulation. Effects on interneuron development are further suggested, in males, by changes in Nrg1 and Erbb4 expression controlling interneuron migration. Our investigation disclosed genes involved in cell-autonomous regulation of developmental processes as potential targets of endocrine disrupters. If corroborated by additional studies, they may serve as markers of early developmental effects in regulatory test development.

2021-11-08 | GSE180054 | GEO

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