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ABSTRACT: metagenomic analysis of shrimp in Shenzhen

PROVIDER: PRJNA1067835 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR27713665_1.fastq.gz	Fastqsanger.gz
	SRR27713665_2.fastq.gz	Fastqsanger.gz
	SRR27713666_1.fastq.gz	Fastqsanger.gz
	SRR27713666_2.fastq.gz	Fastqsanger.gz
	SRR27713667_1.fastq.gz	Fastqsanger.gz

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Project description:Shrimp allergy is the second most common food allergy in the United States. γδ T cells play a regulatory role in peanut immunotherapy, but their role in shrimp allergy remains unclear. We hypothesized γδ T cells play a regulatory role in shrimp allergic disease. We performed single cell RNA sequencing on peripheral cells from shrimp allergic (SA) and healthy control (HC) subjects after stimulation with shrimp tropomyosin. We found significant expansion of γδ T cells and three distinct clusters. One γδ T cell cluster predominated in SA, characterized as CD8+ with a cytotoxic expression profile. We found significant upregulation of TGF-β1 and downregulation of IL-7R in SA-stimulated vs. HC-stimulated γδ T cells, and IL-10 secretion in stimulated SA γδ T cells. γδ T cells play an important role in the pathogenesis of shrimp allergy through lymphocyte-mediated cytotoxin signaling and cytokine-mediated signaling pathways, including TGFβ-1, IL7/TSLP-IL7R, and IL10-IL10R pathways.

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