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Conserved dorsal horn neuron subtype-specific enhancers are implicated in the genetic risk of chronic pain [Mouse Xenium]

ABSTRACT: Conserved dorsal horn neuron subtype-specific enhancers are implicated in the genetic risk of chronic pain [Mouse Xenium]

PROVIDER: PRJNA1067992 | ENA |

REPOSITORIES: ENA

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Conserved dorsal horn neuron subtype-specific enhancers are implicated in the genetic risk of chronic pain [Mouse Xenium]

Project description:The dorsal horn of the spinal cord transforms incoming somatosensory information and transmits it supraspinally to generate sensory perception, including pain and itch. Recent research using mouse Cre-driver lines has implicated specific populations of dorsal horn neurons in the transmission of different types of pain. In parallel, human genome-wide association studies (GWAS) have identified dozens of loci confidently associated with the genetic predisposition to chronic pain. The ability to connect controlled experiments in rodent models with human genetic studies could provide a platform for translational research, but the cell type heterogeneity of the dorsal horn and the complex genetic architecture of chronic pain have created challenges in bridging that gap. Here, we apply a variety of single cell genomic technologies and a comparative genomic analysis to identify conserved dorsal horn neuron subtypes whose open chromatin regions show enrichment for genetic variants associated with human chronic pain phenotypes. To achieve this, we first use single nucleus RNA-Seq and fluorescence in situ hybridization in Rhesus macaque to create a more detailed map of primate dorsal horn neuron subtypes. These were integrated with publicly available human and mouse single nucleus RNA-Seq datasets to create a multi-modal cross species atlas. Then, for the mouse dorsal horn, we combined single nucleus RNA-Seq, spatial transcriptomics, and single nucleus ATAC-Seq to infer spatial and epigenomic profiles of conserved dorsal horn neuron subtypes. Finally, we compared our conserved cell-type open chromatin resource to chronic pain GWAS and found that open chromatin regions of specific dorsal horn neuron subtypes showed enrichment for a variety of human chronic pain phenotypes. Our results provide a foundation to further explore how conserved dorsal horn neuron subtypes influence the transmission of pain signals.

2024-08-01 | GSE253951 | GEO

Conserved dorsal horn neuron subtype-specific enhancers are implicated in the genetic risk of chronic pain [Macaque snRNA-seq]

2024-08-01 | GSE253953 | GEO

Conserved dorsal horn neuron subtype-specific enhancers are implicated in the genetic risk of chronic pain [Mouse snATAC-seq]

2024-08-01 | GSE253952 | GEO

Epitranscriptomic profiling of N4-acetylcytidine-related RNA acetylation in spinal dorsal horn of rat with cancer-induced bone pain

Project description:To evaluate ac4C acetylation in the spinal dorsal horn of rats with bone cancer pain.

2023-05-23 | GSE225571 | GEO

Conserved dorsal horn neuron subtype-specific enhancers are implicated in the genetic risk of chronic pain.

Project description:Conserved dorsal horn neuron subtype-specific enhancers are implicated in the genetic risk of chronic pain.

| PRJNA1067985 | ENA

Transcription profiling of rat adult and neonate dorsal horn after sciatic nerve injury

Project description:As rats do not develop neuropathic pain like hypersensitivity as neonates post nerve injury but do as adults we have used these arrays to help define the processes involved in this process. Rat spinal cord (ipsilateral dorsal horn) was assayed 7 days post SNI injury to the sciatic nerve relative to sham injury. Two age groups of animals were tested Neonates (P10) and Adult (8-12wks). Experiment Overall Design: Six biologically indepenedent arrays were hybridized per assay point. Dorsal horn total RNA was prepared using standard Affymetrix protocols. Affymetrix Rat Expression 230A array used.

2009-11-10 | E-GEOD-18803 | biostudies-arrayexpress

mRNA expression of spinal dorsal horn in oxaliplatin-treated rats

Project description:Oxaliplatin-induced neuropathic pain is a common dose-limiting side effect of cancer treatment but the underlying mechanisms are largely unknown. The neuropathic pain model was established by oxaliplatin intraperitoneal administration for five consecutive days. In the present study, we performed whole genome expression microarray analysis by using spinal dorsal horn from oxaliplatin-treated and vehicle-treated rats on day 10.

2020-03-11 | GSE146739 | GEO

Conserved dorsal horn neuron subtype-specific enhancers are implicated in the genetic risk of chronic pain [Macaque snRNA-seq]

Project description:Conserved dorsal horn neuron subtype-specific enhancers are implicated in the genetic risk of chronic pain [Macaque snRNA-seq]

| PRJNA1067990 | ENA

Conserved dorsal horn neuron subtype-specific enhancers are implicated in the genetic risk of chronic pain [Mouse snATAC-seq]

Project description:Conserved dorsal horn neuron subtype-specific enhancers are implicated in the genetic risk of chronic pain [Mouse snATAC-seq]

| PRJNA1067989 | ENA

Quantitative characterization of the neuropeptide level changes in dorsal horn and dorsal root ganglia regions of the murine itch models

Project description:In this study, we performed a label-free relative quantitation to identify the peptide candidates correlated with itch sensation in murine models. We tested two different itch models-the AEW model (known commonly as the dry skin model) and the MC903 model. Performed peptide extraction from the dorsal horn (DH) and dorsal root ganglia regions of these two models and compared their levels with that of control mice.

2020-01-23 | PXD015949 | Pride

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