Project description:Culex pipiens molestus and Cx. p. quinquefasciatus are the members of Culex pipiens Complex, but they display relatively large differences in behavior and physiological responses. We compared the genes of these mosquitoes to identify those that were differentially expressed in each subspecies. Such genes could play important roles in subspecies-specific blood feeding or oviposition behavior. Culex pipiens molestus and Cx. p. quinquefasciatus females were undertaken Illumina RNA sequencing.
Project description:Culex pipiens molestus and Cx. p. quinquefasciatus are the members of Culex pipiens Complex, but they display relatively large differences in behavior and physiological responses. We compared the genes of these mosquitoes to identify those that were differentially expressed in each subspecies. Such genes could play important roles in subspecies-specific blood feeding or oviposition behavior.
Project description:The following dataset is a Wolbachia proteome derived from protein extractions of infected ovaries of the mosquito host Culex pipiens.
Project description:Investigation of gene expression level differences in Culex pipiens (SB) and C. quinquefasciatus (JHB) at three time points (8, 16, and 24 hours post-exposure) during the early pupal state in standard (25°C; 16 h light/8 h dark) and diapause-inducing (18°C; 8 h light/16 h dark) conditions. A forty-eight chip study (4 Expr12x135K slides) using cDNA from total RNA collected from two species in the Culex pipiens complex at 3 time-points during the early pupal stage to study gene expression differences between standard (25°C; 16 h light/8 h dark) and diapause-inducing (18°C; 8 h light/16 h dark) conditions, and between Culex pipiens (diapause) and C. quinquefasciatus (no diapause). Each chip measures the expression level of 18,692 protein coding genes, with 3 probes per gene and two-fold technical redundancy. Each of the 12-plex slides was used for one of four biological replicates. Probes were designed using the C.quinquefasciatus CpipJ1.2 geneset in VectorBase.
Project description:A protein pilot dataset detecting Wolbachia proteins from protein extracted from dissected infected Culex pipiens mosquito ovaries. The experiment was based of an iTRAQ experiment comparing infected and uninfected ovarian tissues and has been usefull in characterizing the wPip (Buckeye) ovarian proteome.
2017-03-06 | PXD004047 | Pride
Project description:PipPop - the Culex pipiens population genomics project
Project description:A pyrethroid-resistant strain of Culex quinquefasciatus, JPal-per, exhibits 2500-fold greater larval resistance to permethrin than the insecticide-susceptible strain Ogasawara. An increased microsome monooxygenase metabolism is involved in the resistance mechanism. Microarray analysis revealed altered expressions of cytochrome P450 genes in the fourth instar larvae of JPal-per compared to those in OGS. An oligo DNA array was designed for 62 different cDNA segments encoding unique P450 isoforms of the Cx. pipiens complex. Other probes for non-P450 Cx. pipiens complex genes were also mounted on the array.
Project description:Investigation of gene expression level differences in Culex pipiens (SB) and C. quinquefasciatus (JHB) at three time points (8, 16, and 24 hours post-exposure) during the early pupal state in standard (25°C; 16 h light/8 h dark) and diapause-inducing (18°C; 8 h light/16 h dark) conditions.
Project description:A pyrethroid-resistant strain of Culex quinquefasciatus, JPal-per, exhibits 2500-fold greater larval resistance to permethrin than the insecticide-susceptible strain Ogasawara. An increased microsome monooxygenase metabolism is involved in the resistance mechanism. Microarray analysis revealed altered expressions of cytochrome P450 genes in the fourth instar larvae of JPal-per compared to those in OGS. An oligo DNA array was designed for 62 different cDNA segments encoding unique P450 isoforms of the Cx. pipiens complex. Other probes for non-P450 Cx. pipiens complex genes were also mounted on the array. Two strains with two color experiment, total 10 replicates, 5 biological replicates (each strain), 5 dye-swap experiments.
