Project description:The plant hormone jasmonic acid (JA) plays a central role in plant defense responses.12-oxo-phytodienoic acid (OPDA) reductase 3 (OPR3) is a key enzyme in the JA synthesis pathway.To discover what facilitates defense functions in the JA pathway, we conducted a microarray analysis of OPR3 overexpressing rice plants (OPR3ox) and the wild-type Zhonghua 11.
Project description:Jasmonic acid (JA) is involved in various developmental processes and defense responses against abiotic and biotic stresses. We identified JA-responsive genes in rice leaves 6-48 h after treatment using rice 44k microarray.
Project description:Jasmonic acid (JA) is involved in various developmental processes and defense responses against abiotic and biotic stresses. We identified JA-responsive genes in suspension-cultured rice cells 2-12 h after treatment using rice 44k microarray.
Project description:Jasmonates is inductively produced as a major plant hormone responsible for defense reactions in plants against both biotic and abiotic stresses, such as pathogen infection and mechanical wounding. We identified JA-inducible genes in the wild-type rice leaves 0 - 4 h after JA treatment using 44k microarray. Expression profiling in the wild-type rice leaves treated with jasmonic acid for 0.5, 1, 2, and 4 h was compared with that in the untreated wild-type rice leaves using two-color method with three biological replicates.
Project description:Autotoxicity plays an important mechanism in regulating plant productivity. Ferulic acid (FA) is phytotoxic and was identified in extracts and residues of rice plants as a candidate for rice allelochemicals. To help characterize the autotoxicity mechanism of rice, we present the first large-scale, transcriptomic analysis of rice root responses to ferulic acid.
Project description:Jasmonates is inductively produced as a major plant hormone responsible for defense reactions in plants against both biotic and abiotic stresses, such as pathogen infection and mechanical wounding. Jasmonoyl isoleucine is known to be a bioactive compound of jasmonate and plays a pivotal role for plant defenses. We identified OsJAR1M-bM-^HM-^Rrelated JA-inducible genes in osjar1 tos17 mutant (osjar1-2) rice leaves 0 - 2 h after JA treatment using 44k microarray. Expression profiling in the wild-type rice leaves treated with jasmonic acid for 0, 0.5, 1, and 2 h was compared with that in the osjar1 mutant leaves treated with jasmonic acid for 0, 0.5, 1, and 2 h using two-color method with three biological replicates.
Project description:Jasmonic acid (JA) is involved in various developmental processes and defense responses against abiotic and biotic stresses. We identified JA-responsive genes in rice leaves 6-48 h after treatment using rice 44k microarray. Expression profiling in rice leaves treated with JA for 6, 12, 24 and 48 h was compared with that in the corresponding mock control using two-color method with two biological replicates.
Project description:Jasmonic acid (JA) is involved in various developmental processes and defense responses against abiotic and biotic stresses. We identified JA-responsive genes in suspension-cultured rice cells 2-12 h after treatment using rice 44k microarray. Expression profiling in rice cells treated with JA for 2, 4, 6, 8 and 12 h was compared with that in untreated control using two-color method with two biological replicates.
Project description:To understand the dynamics and global gene reprogramming in the early response to mechanical wounding in rice, the transcriptional response to mechanical injury was analyzed. A time-course experiment revealed the highly dynamic nature of the wound response in rice. Mechanical wounding triggered extensive gene expression reprogramming in the locally wounded leaf, affecting various physiological processes, including defense mechanisms and potentially tissue repair and regeneration. The rice response to mechanical wounding displayed both differences and similarities compared to the response to jasmonate treatment. These results highlight the importance of early JA signaling in response to mechanical stress in rice. This analysis provides an overview of the global transcriptional response to mechanical stress in rice, offering valuable insights for future studies on rice's response to injury, insect attack, and abiotic stresses.