Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Central regulation of feeding and body weight by ciliary GPR75

ABSTRACT: Central regulation of feeding and body weight by ciliary GPR75

PROVIDER: PRJNA1069062 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR27730855_1.fastq.gz	Fastqsanger.gz
	SRR27730855_2.fastq.gz	Fastqsanger.gz
	SRR27730856_1.fastq.gz	Fastqsanger.gz
	SRR27730856_2.fastq.gz	Fastqsanger.gz
	SRR27730857_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 24

Similar Datasets

Central regulation of feeding and body weight by ciliary GPR75

Project description:Variants of the G protein-coupled receptor 75 (GPR75) are associated with lower BMI in large-scale human exome sequencing studies. However, how GPR75 regulates body weight remains poorly understood. Using random germline mutagenesis in mice, we identified a missense allele (Thinner) of Gpr75 that resulted in a lean phenotype and verified the decreased body weight and fat weight in Gpr75 knockout (Gpr75–/–) mice. Gpr75–/– mice displayed reduced food intake under a high-fat diet (HFD), and pair-feeding normalized their body weight. The endogenous GPR75 protein was exclusively expressed in the brains of 3xFlag tagged Gpr75 knock-in (3xFlag-Gpr75) mice, with consistent expression across different brain regions. GPR75 interacted with Gαq to activate various signaling pathways after HFD feeding. Additionally, GPR75 was localized in the primary cilia of hypothalamic cells, whereas the Thinner mutation (L144P) and human GPR75 variants with lower BMI failed to localize in the cilia. Loss of GPR75 selectively inhibited weight gain in HFD-fed mice but failed to suppress the development of obesity in Leptin ob mice and Adenylate cyclase 3 (Adcy3) mutant mice on a chow diet. Our data reveal that GPR75 is a ciliary protein expressed in the brain and plays an important role in regulating food intake.

2024-08-13 | GSE254239 | GEO

A single-cell transcriptomic atlas of the human ciliary body

Project description:The ciliary body is required for the maintenance of intraocular pressure and immunity as well as vision accommodation. We report a comprehensive cell atlas of human ciliary body from single-cell RNA sequencing (scRNAseq)

2022-10-05 | GSE206026 | GEO

Exploratory Single Cell RNA Sequencing: Murine Ciliary Body Transcriptome

Project description:Single cell RNA-seq was performed on dissected murine ciliary body samples, and the resulted sequencing data was used to assess the various cell types present within the dissected tissue

2021-07-10 | GSE178667 | GEO

Isolating Independent Effects of Diet/Energy Balance and Body Weight on Behavior and Metabolic Health

Project description:Associated with numerous metabolic and behavioral abnormalities, obesity is classified by metrics reliant on body weight (such as body mass index). However, overnutrition is the common cause of obesity, and may independently contribute to these obesity-related abnormalities. The goal of this study is to isolate ‘diet/energy balance’ effects independent from ‘body weight’ effects on various metabolic and behavioral parameters using the Diet Switch feeding paradigm in mice. [We conducted] unbiased gene expression analysis of the nutrient-sensing circumventricular hypothalamus [using RNA-seq]. Remarkably, only two genes responded to diet/energy balance (neuropeptide y [npy] and agouti-related peptide [agrp]), while others were related only to body weight. Furthermore, linear regression models revealed that npy and agrp showed similar modifiability by diet/energy balance and body weight compared to electroencephalographic-measured sleep/wake behavior.

2018-05-17 | GSE104709 | GEO

Effect of selection on residual feed intake on gene expression in porcine liver (115 kg body weight)

Project description:Transcriptional profiling in liver of 24 pigs of 115 kg body weight from lines divergently selected for residual feed intake (RFI): low-RFI pigs fed ad libitum (RFIneg), high-RFI pigs fed ad libitum (RFIpl) and high-RFI pigs restricted at RFIneg feeding level (RFIplR) to investigate the impact of feeding independently of selection.

2014-01-01 | GSE47767 | GEO

Effect of selection on residual feed intake on gene expression in pig subcutaneous adipose tissue (115 kg body weight)

Project description:Transcriptional profiling in subcutaneous adipose tissue of 24 pigs of 115 kg body weight from lines divergently selected for residual feed intake (RFI): low-RFI pigs (RFIneg), high-RFI pigs (RFIpl) and high-RFI pigs restricted at RFIneg feeding level (RFIplR) to investigate the impact of feeding independently of selection.

2014-01-01 | GSE47771 | GEO

Effect of selection on residual feed intake on gene expression in porcine skeletal muscle (115 kg body weight)

2014-01-01 | GSE47769 | GEO

The proteome of normal human iris, ciliary body, retinal pigment epithelium, and choroid

Project description:The iris is a fine structure that controls the amount of light that enters the eye. The ciliary body controls the shape of the lens and produces aqueous humor. The retinal pigment epithelium and choroid (RPE/choroid) are essential in supporting the retina and absorbing light energy that enters the eye. Proteins were extracted from iris, ciliary body, and RPE/choroid tissues of eyes from five individuals and fractionated using SDS-PAGE. After in-gel digestion, peptides were analyzed using LC-MS/MS on an Orbitrap Elite mass spectrometer. In iris, ciliary body, and RPE/choroid, we identified 2,959, 2,867, and 2,755 non-redundant proteins with protein false positive rate <1%. There were 43 unambiguous protein isoforms identified in iris, ciliary body, and RPE/choroid. Four “missing proteins” were found in ciliary body. The MS proteome database of the human iris, ciliary body, and RPE/choroid may serve as a valuable resource for future investigations of the eye in health and disease. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD001424.

2017-11-29 | MSV000081766 | MassIVE

The proteome of normal human iris, ciliary body, retinal pigment epithelium, and choroid

2016-02-04 | PXD002194 | Pride

Exploratory Single Cell RNA Sequencing: Murine Ciliary Body Transcriptome

Project description:Exploratory Single Cell RNA Sequencing: Murine Ciliary Body Transcriptome

| PRJNA739981 | ENA