Transcriptional profiling of spleens from mice challenged with Brucella melitensis with or without prior vaccination.
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1069075 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR27731323_1.fastq.gz | Fastqsanger.gz | |||
| SRR27731323_2.fastq.gz | Fastqsanger.gz | |||
| SRR27731324_1.fastq.gz | Fastqsanger.gz | |||
| SRR27731324_2.fastq.gz | Fastqsanger.gz | |||
| SRR27731325_1.fastq.gz | Fastqsanger.gz |
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Project description:We isolated RNA from whole spleens of mice challenged with B. melitensis, some of which were vaccinated prior to infection.
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Project description:In this study we attempt to elucidate some of the pathways involved in the immune response to vaccination and subsequent disease challenge using a transcriptomic approach. We exposed, via intra-peritoneal injection, three months old Asian seabass (Lates calcarifer) to a Streptococcus iniae vaccine. A control group was also set up where no vaccine was injected. Spleen and head kidney samples were collected at one and seven days post vaccination for transcriptomic analysis. At this point, there are four groups per organ: Day 1 vaccinated, Day 1 control, Day 7 vaccinated and Day 7 control. Subsequently, a pathogen challenge was carried out three weeks later and spleen and head kidneys were sampled at 25-29 hours post challenge for transcriptomic analysis. For control, mock challenged was carried out. At this point, there are four groups per organ: unvaccinated challenged, unvaccinated mock challenged, vaccinated challenged and vaccinated mock challenged.
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