Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Identification of calcineurin-dependent gene expression changes in response to chronic CaCl2 stress

ABSTRACT: Identification of calcineurin-dependent gene expression changes in response to chronic CaCl2 stress

PROVIDER: PRJNA1071032 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Identification of calcineurin-dependent gene expression changes in response to chronic CaCl2 stress

Project description:Gene expression was quantified in S. cerevisae cells over a time course of three days of CaCl2 treatment to activate calcineurin. Wild type (WT) and cnb1 mutant strains were compared to allow the identification of calcineurin-dependent gene expression changes. We show that mitochondrial genes are downregulated, and ribosome biogenesis genes are upregulated, in both geneotypes over time.

2024-03-19 | GSE254551 | GEO

Identification of calcineurin-dependent, Crz1-independent gene expression changes in response to chronic CaCl2 stress

Project description:Gene expression was quantified in S. cerevisae cells over a time course of three days of CaCl2 treatment to activate calcineurin. crz1 and crz1 cnb1 mutant strains were compared to allow the identification of calcineurin-dependent gene expression changes that are independent of the downstream transcription factor Crz1. We show that mitochondrial genes are downregulated in both geneotypes over time. Additionally, genes that promote glutathione biosynthesis are upregulated in cells that express calcineurin.

2024-03-19 | GSE254552 | GEO

Identification of calcineurin-dependent, Crz1-independent gene expression changes in response to chronic CaCl2 stress

Project description:Identification of calcineurin-dependent, Crz1-independent gene expression changes in response to chronic CaCl2 stress

| PRJNA1071031 | ENA

Identification of calcineurin-dependent gene expression changes in response to chronic stress

Project description:Identification of calcineurin-dependent gene expression changes in response to chronic stress

| PRJNA1071023 | ENA

Identification of calcineurin-dependent, Crz1-independent gene expression changes in response to chronic KCl stress

Project description:Gene expression was quantified in S. cerevisae cells after 48 hours of treatment with KCl. crz1 and crz1 cnb1 mutant strains were compared to allow the identification of calcineurin-dependent gene expression changes that are independent of the downstream transcription factor Crz1. We find that calcineurin has very little effect on gene expression in response to KCl.

2024-03-19 | GSE254554 | GEO

Identification of calcineurin-dependent, Crz1-independent gene expression changes in response to chronic KCl stress

Project description:Identification of calcineurin-dependent, Crz1-independent gene expression changes in response to chronic KCl stress

| PRJNA1071034 | ENA

Dynamics of calcineurin-regulated gene expression in S. cerevisiae

Project description:Gene expression was quantified in crz1 delete S. cerevisae cells over a time course following calcineurin activation by CaCl2. Cells were pretreated with buffer or the calcineurin inhibitor FK506 to allow the identification of calcineurin-regulated genes. A strain with a calcineurin-resistant mutation in the transcription factor Hcm1 was also examined. We show that calcineurin activation leads to downregulation of a many clusters of cell cycle-regulated genes, including those expressed at the G1/S and G2/M transitions.

2020-01-14 | GSE115013 | GEO

Dynamics of calcineurin-regulated gene expression in the absence of Hog1 in S. cerevisiae

Project description:Gene expression was quantified in crz1 hog1 delete S. cerevisae cells over a time course following calcineurin activation by CaCl2. Cells were pretreated with buffer or the calcineurin inhibitor FK506 to allow the identification of calcineurin-regulated genes. We show that calcineurin-dependent downregulation of G1/S genes is partly dependent on the osmostress-activated kinase Hog1.

2020-01-14 | GSE115014 | GEO

Ca2+/calcineurin/CRZ1 dependent gene expression in Magnaporthe oryzae

Project description:The Ca2+/calcineurin signaling pathway is a central conduit regulating growth, development, and virulence of fungal pathogens infecting plants and human. We have analyzed global gene expression profiles during Ca2+ treatment in the rice blast fungus, Magnaporthe oryzae. An immunosuppressive drug, FK506, and the knock-out mutant of a transcription factor, MoCRZ1, were included to analyze calcineurin- and/or CRZ1-dependent gene expression, respectively. About 1,400 genes were up or down regulated by Ca2+ treatment, while about 200 genes seemed to be up-regulated in a calcineurin/CRZ1-dependent manner. This study analyzes global gene expression dynamics during Ca2+ treatment in the rice blast fungus. We have used Agilent M. grisea 2.0 4×44K Microarrays using a single channel hybridization design. An immunosuppressive drug, FK506, and the knock out mutant of a transcription factor, MoCRZ1, were included to analyze calcineurin- and/or CRZ1-dependent gene expression, respectively. To know the time point at which the gene expression fluctuates most significantly, we treated mycelia with CaCl2 for 0, 15, 30, and 60 min. PMC1 expression level was checked by RT-PCR with the highest expression at 30 min time point. Four biological replicates of wild type and mutant mycelia were harvested after 30 min treatment with chemicals. Pairwise comparisons between Ca2+ treated vs. control and Ca2+ vs. Ca2+/FK506 in wild type (WT), and Ca2+ treated in WT vs. in mutant were conducted.

2010-09-17 | E-GEOD-18185 | biostudies-arrayexpress

Identification of calcineurin-dependent gene expression changes in response to chronic stress

Project description:This SuperSeries is composed of the SubSeries listed below.

2024-03-19 | GSE254555 | GEO