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Single-cell RNA sequencing in Kawasaki disease II

ABSTRACT: Single-cell RNA sequencing in Kawasaki disease II

PROVIDER: PRJNA1071263 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR27793192_1.fastq.gz	Fastqsanger.gz
	SRR27793192_2.fastq.gz	Fastqsanger.gz
	SRR27793193.fastq.gz	Fastqsanger.gz
	SRR27793194_1.fastq.gz	Fastqsanger.gz
	SRR27793194_2.fastq.gz	Fastqsanger.gz

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Single-cell RNA sequencing in Kawasaki disease

Project description:Single-cell RNA sequencing in Kawasaki disease

| PRJNA826467 | ENA

Single-cell sequencing of peripheral blood mononuclear cells in acute Kawasaki disease

Project description:Kawasaki disease (KD) is the most common cause of acquired heart disease in children in developed countries. Based on single-cell RNA sequencing (scRNA-seq), we profiled peripheral blood mononuclear cells (PBMCs) from patients with acute KD before and after intravenous immunoglobulin (IVIG) therapy and from healthy controls. We identified the dynamics of cell abundance, gene expression patterns and biological processes underlying the immune dysregulation of each cell compartment. The single-cell landscape of both innate and adaptive immune responses provides new insights into pathogenesis and therapy of KD.

2021-03-12 | GSE168732 | GEO

Single-cell RNA sequencing in Kawasaki disease II

Project description:We performed single-cell RNA sequencing (using Cell Ranger Single Cell Pipeline v.4.0.0 to process data de-multiplexing, barcode processing, and single cell 3' gene counting generated by the 10X Chromium platform) in KD patients (within 24 hours before IVIG treatment) and age-matched fever controls.

2024-06-01 | GSE254657 | GEO

MicroRNA profile of exosome from serum samples of Kawasaki disease

Project description:To further development of our miRNA diagnostic approach to Kawasaki disease(KD), we have employed microRNA microarray expression profiling as a discovery platform to identify microRNAs as the potential biomarkers to rapidly diagnose Kawasaki disease. Pooled exosome of serum in equal amount from 5 healthy children, 5 KD patients and 5 KD patients after Intravenous immunoglobulin (IVIG) therapy were used for microRNA microarray analysis. MicroRNA profile of exosome from Kawasaki disease(KD) was analyzed by microRNA microarray analysis in 5 healthy children, 5 KD patients and 5 KD patients after IVIG therapy.

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Single-cell RNAseq (10x Genomics) analysis of the abdominal aorta in a murine model of Kawasaki disease vasculitis

Project description:Single-cell RNAseq (10x Genomics) analysis of the abdominal aorta in a murine model of Kawasaki Disease vasculitis (Lactobacillus casei cell wall extract, LCWE) and in control PBS-injected mice

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Single-cell sequencing of peripheral blood mononuclear cells in acute Kawasaki disease

Project description:Single-cell sequencing of peripheral blood mononuclear cells in acute Kawasaki disease

| PRJNA713790 | ENA

MicroRNA profile of exosome from serum samples of Kawasaki disease

2014-09-03 | GSE60965 | GEO

Kawasaki Disease and Other Febrile Illnesses

Project description:ABSTRACT Background. Acute Kawasaki disease (KD) is difficult to distinguish from other acute rash/fever illnesses, in part because the etiologic agent(s) and pathophysiology remain poorly characterized. As a result, diagnosis and critical therapies may be delayed. Methods. We used DNA microarrays to identify possible diagnostic features of KD. We compared gene expression patterns in the blood of 23 children with acute KD and 18 age-matched febrile children with three illnesses that resemble KD. Results. Genes associated with platelet and neutrophil activation were expressed at higher levels in KD patients than in patients with acute adenovirus infections or systemic adverse drug reactions but not in patients with scarlet fever; genes associated with B cell activation were also expressed at higher levels in KD patients than in controls. A striking absence of interferon-stimulated gene expression in the KD patients was confirmed in an independent cohort of KD subjects. We successfully predicted the diagnosis in 21 of 23 KD patients and 7 of 8 adenovirus patients using a set of 38 gene transcripts. Conclusions. These findings provide insight into the molecular features that distinguish KD from other febrile illnesses, and support the feasibility of developing novel diagnostic reagents for KD based on the host response. A disease state experiment design type is where the state of some disease such as infection, pathology, syndrome, etc is studied. Disease State: One of Kawasaki Disease (KD) or control (C) of Scarlet fever (C-sf), adenovirus infection (C-ai) or drug reaction (C-dr) disease_state_design

2010-06-24 | E-GEOD-15297 | biostudies-arrayexpress

Single-cell RNA sequencing in Kawasaki disease

2022-09-06 | GSE200743 | GEO

Transcription profiling of Kawasaki disease patients who may benefit from methylprednisolone

Project description:Clinical score and transcript abundance patterns identify Kawasaki disease patients who may benefit from addition of methylprednisolone. Intravenous immunoglobulin (IVIG) treatment-resistant patients are high risk of developing coronary artery lesions (CALs) with Kawasaki disease (KD). The IVIG-responsive (Group A; n = 6) and -resistant patients (Group B) were predicted before starting the initial treatment using the Egami scoring system, and randomly allocated a single-IVIG treatment group (Group B1; n = 6) or a IVIG-plus-methylprednisolone (IVMP) combined therapy group (Group B2; n = 5). We investigated transcript abundance in the leukocytes of those patients using microarray analysis. Results: five patients in Group A and 1 patient in Group B1 responded to initial IVIG treatment. All Group B2 patients responded to IVIP-plus-IVMP combined therapy. Prior to performing these treatments, those transcripts related to IVIG-resistance and to the development of CALs, such as IL1R, IL18R, oncostatin M, suppressor of cytokine signaling-3, S100A12 protein, carcinoembryonic antigen-related cell adhesion molecule-1, matrix metallopeptidase-9 and polycythemia rubra vera-1 were more abundant in Group B patients in comparison to Group A patients. Moreover, those transcripts in Group B2 patients were more profoundly and broadly suppressed than Group B1 patients after treatment. Conclusion: this study elucidated the molecular mechanism of the effectiveness of IVIG-plus-IVMP combined therapy. 34 samples of pre- and post-treatment in three groups consisting of predicted as IVIG-responsive patients, given single-IVIG treatment patients and IVIG-plus-IVMP combined therapy group in predicted as IVIG-resistant patients.

2010-07-08 | E-GEOD-16797 | biostudies-arrayexpress

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