Project description:The goal of the experiment – genome-wide profiling of DNA methylation reveals a class of normally methylated CpG island promoters Keywords: DNA methylation, Methylated CpG island amplification coupled with promoter arrays, normal tissue
Project description:SORT-seq is a plate-based method of single-cell RNA sequencing (Muraro et al. 2016) is a partially robotized version of the CEL-seq2 protocol (Hashimshony et al. 2016). Synovial membranes from Rheumatoid arthritis' patients yield a relatively robust number of DCs using scRNAseq, we sought to validate this initial finding at protein level. We employed a rigorous gating strategy to sort specific ST DC subsets, developed based on CITEseq analysis, which was then confirmed by index cell plate sort sequencing (SORT-seq).
Project description:Nucleoid structuring protein H-NS silences the expression of Salmonella Pathogenicity Island 1 (SPI-1) by binding to the promoter region of SPI-1's master regulator. The experiment aims at testing whether transcription of sequences more than one Kb upstream of the regulator gene causes H-NS:DNA complexes throughout SPI-1 (and elsewhere) to disassemble (as suggested by functional assays).