ATAC-sequencing of Artemia cyst at 30min after embryonic diapause termination
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PROVIDER: PRJNA1072660 | ENA |
REPOSITORIES: ENA
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Project description:Embryonic diapause is a widely occurring evolutionary adaptation phenomenon in animals. Artemia is one of the classic animal models for diapause research. The current studies of Artemia diapause mainly focus on the induction and maintenance of the embryonic diapause, but there is little research on the molecular regulatory mechanism of Artemia embryonic diapause termination (EDT) and embryonic reactivation. Here the gene expression of Artemia cyst at 30min after embryonic diapause termination (EDT), which is in post-diapause stage were tested by RNA-seq to analyze the mechanism of signal regulation involved in Artemia EDT at the molecular level.
2024-05-01 | GSE254935 | GEO
Project description:Embryonic diapause is a widely occurring evolutionary adaptation phenomenon in animals. Artemia is one of the classic animal models for diapause research. The current studies of Artemia diapause mainly focus on the induction and maintenance of the embryonic diapause, but there is little research on the molecular regulatory mechanism of Artemia embryonic diapause termination (EDT) and embryonic reactivation. Here the gene expression of Artemia cyst at 30min after embryonic diapause termination (EDT), which is in post-diapause stage were tested by ATAC-seq to analyze the mechanism of signal regulation involved in Artemia EDT at the molecular level.
2024-05-01 | GSE254934 | GEO
Project description:Embryonic diapause is a widely occurring evolutionary adaptation phenomenon in animals. Artemia is one of the classic animal models for diapause research. The current studies of Artemia diapause mainly focus on the induction and maintenance of the embryonic diapause, but there is little research on the molecular regulatory mechanism of Artemia embryonic diapause termination (EDT) and embryonic reactivation. Here the gene expression of Artemia cyst in diapause stage and 5 hours after embryonic diapause termination (EDT), which is in post-diapause stage were tested by RNA-seq to analyze the mechanism of signal regulation involved in Artemia EDT at the molecular level.
2024-05-01 | GSE249417 | GEO
Project description:Embryonic diapause is a widely occurring evolutionary adaptation phenomenon in animals. Artemia is one of the classic animal models for diapause research. The current studies of Artemia diapause mainly focus on the induction and maintenance of the embryonic diapause, but there is little research on the molecular regulatory mechanism of Artemia embryonic diapause termination (EDT) and embryonic reactivation. Here the gene expression of Artemia cyst in diapause stage and 5 hours after embryonic diapause termination (EDT), which is in post-diapause stage were tested by ATAC-seq to analyze the mechanism of signal regulation involved in Artemia EDT at the molecular level.
2024-05-01 | GSE248452 | GEO
Project description:Transcriptome profiling of Artemia cyst in 30min after embryonic diapause termination
| PRJNA1072659 | ENA
Project description:ATAC-sequencing of Artemia cyst in in diapause stage and 5 hours after embryonic diapause termination
| PRJNA1044056 | ENA
Project description:Transcriptome profiling of Artemia cyst in in diapause stage and 5 hours after embryonic diapause termination
| PRJNA1049008 | ENA
Project description:The most common ladybird beetle, Coccinella septempunctata L., is an excellent predator of crop pests such as aphids and white flies, and it shows a wide range of adaptability, a large appetite and a high reproductive ability. In this study, we collected female adults in three different states, i.e., non-diapause, diapause and diapause termination, for transcriptome sequencing. The experimental insects consisted of three different states as follows: Non-diapause female insects were reared at 24±1°C, with a RH of 70±10% and a 16:8 h light: dark (L: D) photoperiod and collected after their first oviposition. Female adults in diapause were reared at 18±1°C at an RH of 70±10% and a 10:14-h (L:D) photoperiod. The experimental diapause insects were collected after 30 days. Diapause-terminated adults were transferred to another climatic cabinet with the 30-day diapause insects and reared under the same conditions as the non-diapause insects. After their first oviposition, the female insects were collected and stored at -80°C. Three biological replicates per treatment (non-diapause, diapause, diapause-terminated) were sequenced using Illumina HiSeq 2500.
2016-06-01 | E-GEOD-75645 | biostudies-arrayexpress
Project description:Gene expression of two populations was compared at a daylength intermediate to the two population critical photoperiods after 0 and 6 days. Genes with interaction terms are candidates for involvement in long-day response. The mosquito Wyeomyia smithii overwinters in a larval diapause that is initiated, maintained and terminated by day length (photoperiod). We use a forward genetic approach to investigate covert transcriptional events involved in the termination of diapause following exposure to long-days. We incorporate a novel approach that compares two populations (DB - southern, DR - northern) that differentially respond to a single day length. After six long days, 50% of individuals of population DB have terminated diapause and are irrevocably committed to development, though no phenotyping differences are observed. We can compare gene expression between these two populations after six long days and zero long days. The zero long day treatment will control for evolved differences between the populations and the 6 long day treatment allows for the identification of genes that are differentially expressed due to different responses to a single daylength. We identify 30 transcripts associated with differential response to day length. All of the corresponding genes with a previously annotated function are consistent with a role in the termination of diapause, with downstream developmental events, or with the transition from potentially oxygen-poor to oxygen-rich environments; none appears to be specifically part of the photoperiodic switch mechanism itself. However, among 10 unannotated genes, a gene homologous to Drosophila melanogaster CG13043 emerges from three separate forward genetic screens as a leading candidate for a gene contributing to the photoperiodic timing mechanism itself (photoperiodic switch). We name this gene photoperiodic response gene 1 (prg1). Prg1 is up-regulated under long-day response conditions, is located under a QTL for critical photoperiod and is associated with critical photoperiod after 25 generations of recombination from a cross between extreme phenotypes. Three independent forward genetic approaches identify prg1 as a gene either involved in the photoperiodic switch mechanism or very tightly linked to a gene that is. We conclude that continued forward genetic approaches will be central to understanding not only the molecular basis of photoperiodism and diapause, but also the evolutionary potential of temperate and polar animal populations when confronted with rapid climate change. 4 treatments, (Population DR, Day 0; Population DB, Day 0; Population DR, Day 6, Population DB, Day6), one dye swap (treatment Cy3 or Cy5, each replicated three times for a total of 24 arrays
2010-05-14 | E-GEOD-18848 | biostudies-arrayexpress
Project description:The most common ladybird beetle, Coccinella septempunctata L., is an excellent predator of crop pests such as aphids and white flies, and it shows a wide range of adaptability, a large appetite and a high reproductive ability. In this study, we collected female adults in three different states, i.e., non-diapause, diapause and diapause termination, for transcriptome sequencing.
2016-06-01 | GSE75645 | GEO