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Transcriptional profiling of lungs from SARS-CoV-2-infected K18-hACE2 mice

ABSTRACT: Transcriptional profiling of lungs from SARS-CoV-2-infected K18-hACE2 mice

PROVIDER: PRJNA1072723 | ENA |

REPOSITORIES: ENA

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Transcriptional profiling of lungs from SARS-CoV-2-infected K18-hACE2 mice

Project description:To explore the effects of SARS-CoV-2 proteins on expression of host genes in lung of infected mice, 4 groups of K18-hACE2 mice (4 mice per group) were mock-infected or infected cells with SARS-CoV-2 WT or its NPS1, or NSP15 mutants respectively.The mice were euthanized at 4 days post-infection to collect the lungs to isolate RNA for RNA sequencing.

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Delta (B1.617.2) variant of SARS-CoV-2 induces severe neurotropic patterns in K18-hACE2 mice

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Administration of aerosolized SARS-CoV-2 to K18-hACE2 mice uncouples respiratory infection from fatal neuroinvasion

Project description:The development of tractable animal models that faithfully reproduce COVID-19 pathogenesis would arguably be a major benefit. Infection of transgenic mice expressing the human version of the SARS-CoV-2 receptor (hACE2) by intranasal instillation of a liquid SARS-CoV-2 suspension under deep anesthesia results in disproportionate high CNS infection leading to fatal encephalitis, which is rarely observed in humans and severely limits this model’s usefulness. Here, we describe the characterization of a novel COVID-19 mouse model based on an inhalation tower system that allows to expose unanesthetized mice to aerosolized virus under controlled conditions. Aerosol exposure of K18-hACE2 transgenic mice to SARS-CoV-2 resulted in robust viral replication in the respiratory tract, anosmia, and airway obstruction, but did not lead to fatal viral neuroinvasion. When compared to intranasal inoculation, aerosol infection resulted in a more pronounced lung pathology including increased immune infiltration, fibrin deposition and a transcriptional signature comparable to that observed in humans. This model may prove useful for studies of viral transmission, disease pathogenesis (including long-term consequences of SARS-CoV-2 infection) and therapeutic interventions.

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Transcriptome profiling of the lungs of K18-hACE2 mice infected with SARS-CoV-2 wild-type strain or beta variant

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Modelling SARS-CoV-2 Infection in Mice Using Lentiviral hACE2 Vectors Suggests Two Modes of Immune Responses to SARS-CoV-2 Infection

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mouse metagenome strain:K18-hACE2

Project description:Mouse metagenome strain:K18-hACE2 Raw sequence reads

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