Project description:Investigate the effects loss of skeletal muscle Bmal1 has on the acute transcriptional response to exercise in gastrocnemius muscle.
Project description:Investigate the effects loss of skeletal muscle Bmal1 has on systemic transcriptomes +/- exercise training. Mouse liver, heart, white adipose and lung tissues were collected 47 hours post their last exercise bout, with or without 6-weeks of daily treadmill training. +/- Skeletal muscle Bmal1
Project description:TMT analysis of proteomic changes in the gastrocnemius skeletal muscles of WT and Bmal1-KO mice, and Bmal1-KO mice rescued with AAV-mediated muscle-specific expression of Bmal1.
Project description:We generated a tamoxifen-inducible skeletal muscle-specific Bmal1 knockout mouse model and performed a time-course microarray experiment to identify gene expression changes downstream of the molecular clock. We report the transcrpt expression profiles in adult gastrocnemius skeletal muscle harvested from iMS-Bmal1-/- and at 4 hour intervals for a full circadian time-course (6 time-points).
Project description:The goal of this study was to define the binding sites of the core circadian factors, BMAL1 and CLOCK across the genome in adult mouse skeletal muscle. We found that mitochondrial inner-membrane genes were among the most enriched rhythmic genes via gene ontology analysis, and ChIP-Seq analysis corroborated the role of Clock and Bmal1 in targeting promoters of mitochondrial inner-membrane genes.