Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The small protein YjiS acts as a virulence suppressor in Salmonella Typhimurium

ABSTRACT: The small protein YjiS acts as a virulence suppressor in Salmonella Typhimurium

PROVIDER: PRJNA1075134 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR27932100.fastq.gz	Fastqsanger.gz
	SRR27932101.fastq.gz	Fastqsanger.gz
	SRR27932102.fastq.gz	Fastqsanger.gz
	SRR27932103.fastq.gz	Fastqsanger.gz
	SRR27932104.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 24

Similar Datasets

The small protein YjiS acts as a virulence suppressor in Salmonella Typhimurium

Project description:Bacterial small proteins impact diverse physiological processes, however, technical challenges posed by small size hampered their systematic identification and biochemical characterization. In our quest to uncover small proteins relevant for Salmonella pathogenicity, we previously identified YjiS, a 54 amino acid protein, which is strongly induced during this pathogen’s intracellular infection stage and exhibits features of a virulence suppressor. Here, we set out to further characterize the virulence-related role of YjiS. Cell culture infection assays with Salmonella mutants lacking or overexpressing YjiS corroborated previous functional genomics data and additionally suggested this small protein to counteract bacterial escape from macrophages. Mutant scanning of the protein’s conserved, arginine-rich DUF1127 domain excluded a major effect of single amino acid substitutions on the infection phenotype. A comparative dual RNA-seq assay uncovered the molecular footprint of YjiS in the macrophage response to infection, with effects related to oxidative stress and endo-/exocytosis. Bacterial cell fractionation experiments demonstrated YjiS to associate with the inner membrane, without being itself membrane-inserted. Accordingly, proteins interacting with YjiS in pull-down experiments were enriched for inner membrane processes. Together, our study points to a role for Salmonella YjiS as a membrane-associated suppressor of pathogen dissemination.

2025-01-15 | GSE255513 | GEO

The small protein YjiS acts as a virulence suppressor in Salmonella Typhimurium

2024-12-12 | PXD048449 | Pride

Mucispirillum schaedleri interferes with Salmonella Typhimurium virulence factor expression

Project description:Mucispirillum schaedleri interferes with Salmonella Typhimurium virulence factor expression

| PRJNA504618 | ENA

Salmonella Typhimurium reprograms macrophage metabolism via T3SS effector SopE2 to promote intracellular replication and virulence

Project description:Salmonella Typhimurium establishes systemic infection by replicating in host macrophages. Here we show that macrophages infected with S. Typhimurium exhibit upregulated glycolysis and decreased serine synthesis, leading to accumulation of glycolytic intermediates. The effects on serine synthesis are mediated by bacterial protein SopE2, a type III secretion system (T3SS) effector encoded in pathogenicity island SPI-1. The changes in host metabolism promote intracellular replication of S. Typhimurium via two mechanisms: decreased glucose levels lead to upregulated bacterial uptake of 2- and 3-phosphoglycerate and phosphoenolpyruvate (carbon sources), while increased pyruvate and lactate levels induce upregulation of another pathogenicity island, SPI-2, known to encode virulence factors. Pharmacological or genetic inhibition of host glycolysis, activation of host serine synthesis, or deletion of either the bacterial transport or signal sensor systems for those host glycolytic intermediates impairs S. Typhimurium replication or virulence.

2024-05-22 | MTBLS2347 | MetaboLights

Expression analysis of Salmonella enterica serovar Typhimurium UK1 delta-iacP mutant

Project description:Investigation of whole genome gene expression level changes in a Salmonella enterica serovar Typhimurium UK1 delta-iacP mutant, compared to the wild-type strain. IacP is resoponsible for the secretion of virulence effector proteins via the type III secretion system, thereby contributing the virulence of S. Typhimurium. The mutants analyzed in this study are further described in Kim et al. 2011. Role of Salmonella Pathogenicity Island 1 Protein IacP in Salmonella enterica Serovar Typhimurium Pathogenesis. Infection and Immunity 79(4):1440-1450 (PMID 21263021).

2012-04-30 | GSE34663 | GEO

Salmonella Modulates Metabolism during Growth under Conditions that Induce Expression of Virulence Genes

Project description:Salmonella enterica serovar Typhimurium (S. Typhimurium) is a facultative pathogen that uses complex mechanisms to invade and proliferate within mammalian host cells. To investigate possible contributions of metabolic processes to virulence in S. Typhimurium grown under conditions known to induce expression of virulence genes, we used a metabolomics-driven systems biology approach coupled with genome scale modeling. First, we identified distinct metabolite profiles associated with bacteria grown in either rich or virulence-inducing media and report the most comprehensive coverage of the S. Typhimurium metabolome to date. Second, we applied an omics-informed genome scale modeling analysis of the functional consequences of adaptive alterations in S. Typhimurium metabolism during growth under our conditions. Modeling efforts highlighted a decreased cellular capability to both produce and utilize intracellular amino acids during stationary phase culture in virulence conditions, despite significant abundance increases for these molecules as observed by our metabolomics measurements. Furthermore, analyses of omics data in the context of the metabolic model indicated rewiring of the metabolic network to support pathways associated with virulence. For example, cellular concentrations of polyamines were perturbed, as well as the predicted capacity for secretion and uptake.

2014-06-25 | ST000085 | MetabolomicsWorkbench

Effect of ppGpp on Salmonella Typhimurium gene expression

Project description:The expression profile of an S. Typhimurium mutant strain unable to synthesise ppGpp (relAspoT deletions) was compared to the wild-type strain. The effect of ppGpp on virulence gene expression was studied under 4 different growth conditions that induce virulence gene expression. Keywords: genetic modification

2006-05-30 | GSE4631 | GEO

Mucispirillum schaedleri interferes with Salmonella Typhimurium virulence factor expression

Project description:We used transcriptomics to investigate how Mucispirillum schaedleri ASF 457 interferes with the gene expression of Salmonella Typhimurium in the cecum of gnotobiotic mice

2018-11-09 | GSE122312 | GEO

PTIR1 acts as a suppressor of immunoproteasome and promotes cancer immune resistance

Project description:PTIR1 acts as a suppressor of immunoproteasome and promotes cancer immune resistance

| PRJNA714384 | ENA

Expression analysis of Salmonella enterica serovar Typhimurium 14028 delta GidA mutant

Project description:Investigation of whole genome gene expression level changes in a Salmonella enterica serovar Typhimurium 14028 delta GidA mutant The mutant described in this study is further analyzed in Shippy, D. C., N. M. Eakley, P. N. Bochsler, and A. A. Fadl. 2011. Biological and virulence characteristics of Salmonella enterica serovar Typhimurium following deletion of glucose-inhibited division (gidA) gene. Microb Pathog.

2011-07-20 | GSE30787 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data