Project description:E. coli K12 cells (in 9 g/L NaCl) were irradiated with blue laser ligth (448 nm) for 5 s (310 J/cm2) or 20 s (1240 J/cm2) both with an intensity of 62 W/cm2 and the transcriptome was compared to that of unirradiated cells
Project description:The aim of the study was to compare the transcriptome of E. coli K12 MG1655 cells lacking genes known to induce the Rtc RNA repair system, such as gor, mazF and srmB, to wild-type cells. In all cases, less than 1% of the genes were found to be differentially expressed.
Project description:The aim of the study was to compare the transcriptome of E. coli K12 MG1655 cells lacking hydroperoxidase (Hpx-) and therefore unable to detoxify hydrogen peroxide, to wild-type cells; both when the Rtc RNA repair system is active and when it is inhibited; at 8 and 24 hpi. A very large number of genes (up to 1/3) were found to be differentially expressed in Hpx- as compared to wild-type. Inhibition of the Rtc system had dramatic effects on Hpx- but not on wild-type.