Project description:ra07-02_aba2 - maternal/embryonic aba-1 - Identification of genes regulated by maternal and embryonic ABA - Comparison of expression in seeds (15 days after pollination) of aba2-2 mutant to wild type (Col-0), aba2 (female) x Col-0 (male) crossing, and aba2-2 sprayed with ABA (100 micro M, twice a week). Keywords: gene knock out,treated vs untreated comparison
Project description:ra07-02_aba2 - maternal/embryonic aba-1 - Identification of genes regulated by maternal and embryonic ABA - Comparison of expression in seeds (15 days after pollination) of aba2-2 mutant to wild type (Col-0), aba2 (female) x Col-0 (male) crossing, and aba2-2 sprayed with ABA (100 micro M, twice a week). Keywords: gene knock out,treated vs untreated comparison 6 dye-swap - CATMA arrays
Project description:Whether and how organisms can inherit environmental information from their parents is a major question in evolutionary theory. Plants have evolved to link reproductive development to seasonal environmental cues and seed dormancy is highly contingent on the environmental temperature during seed set, although the mechanism by which seeds acquire seasonal timing information is unclear. Here we show that loss of maternal like heterochromatin protein 1 (LHP1) causes an inability of progeny seeds to sense temperature and that this is linked mechanistically to reduced ABA levels in seeds and hyperaccumulation of free nitrate. Remarkably, single cell transcriptomics reveals that in both maternal fruit and seed tissues, the effect of small changes in temperature closely phenocopies the lhp1 mutant phenotype and ABA biosensor imaging reveal large fluxes of maternal ABA into seeds is modulated by temperature cues. We show that temperature activates ABA production in leaves and that maternal ABA is necessary and sufficient for progeny seeds to acquire seed dormancy. Thus, we reveal that the climate experience of mother plants causes adaptation of progeny behaviour via hormone transport during seed set.
Project description:Whether and how organisms can inherit environmental information from their parents is a major question in evolutionary theory. Plants have evolved to link reproductive development to seasonal environmental cues and seed dormancy is highly contingent on the environmental temperature during seed set, although the mechanism by which seeds acquire seasonal timing information is unclear. Here we show that loss of maternal like heterochromatin protein 1 (LHP1) causes an inability of progeny seeds to sense temperature and that this is linked mechanistically to reduced ABA levels in seeds and hyperaccumulation of free nitrate. Remarkably, single cell transcriptomics reveals that in both maternal fruit and seed tissues, the effect of small changes in temperature closely phenocopies the lhp1 mutant phenotype and ABA biosensor imaging reveal large fluxes of maternal ABA into seeds is modulated by temperature cues. We show that temperature activates ABA production in leaves and that maternal ABA is necessary and sufficient for progeny seeds to acquire seed dormancy. Thus, we reveal that the climate experience of mother plants causes adaptation of progeny behaviour via hormone transport during seed set.
Project description:ra14-04_nced - abaseed - Identification of abscisic acid (ABA) signaling factors and targets in Arabidopsis developing seeds by comparing ABA deficient and wild type genotypes. Transcriptome comparison of developing seeds harvested at 14 DAP from two ABA-deficient mutants (nced2 nced5 nced9 and nced2 nced5 nced6 nced9) and wild type (Columbia-0).
Project description:Mature seeds of Arabidopsis thaliana are desiccation tolerant, but they lose DT while progressing to germination. Yet, there is a small developmental window during which DT can be rescued by treatment with abscisic acid (ABA). We used a time-series of microarrays to gain temporal resolution and identify relevant genes in the re-establishment of desiccation tolerance with ABA.
Project description:ra12-02_aba-seed - ra12-02_aba-seed - Identification of ABA signaling factors and targets in Arabidopsis developing seeds by comparing ABA deficient and wild type genotypes - Transcriptome comparison of developing seeds harvested at 10 DAP from two ABA-deficient mutants (nced2 nced5 nced9 and nced2 nced5 nced6 nced9) and wild type (Columbia-0)