Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Identification of genes essential for the cell death induced by OSW-1 using the Genome-wide CRISPR-Cas9 knockout (GeCKO) screening

ABSTRACT: Identification of genes essential for the cell death induced by OSW-1 using the Genome-wide CRISPR-Cas9 knockout (GeCKO) screening

PROVIDER: PRJNA1077328 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR27990760.fastq.gz	Fastqsanger.gz
	SRR27990761.fastq.gz	Fastqsanger.gz
	SRR27990762.fastq.gz	Fastqsanger.gz
	SRR27990763.fastq.gz	Fastqsanger.gz

Items per page:

1 - 4 of 4

Similar Datasets

Genome-wide essential gene identification for lysenin-induced cell death using CRISPR/CAS9 screening system

Project description:Genome-wide essential gene identification for lysenin-induced cell death using CRISPR/CAS9 screening system

| PRJNA769044 | ENA

Genome-wide essential gene identification for Shiga toxin-induced cell death using CRISPR/CAS9 screening system

Project description:Genome-wide essential gene identification for Shiga toxin-induced cell death using CRISPR/CAS9 screening system

| PRJNA480005 | ENA

Genome-wide essential gene identification for SubAB toxin-induced cell death using CRISPR/CAS9 screening system

Project description:Genome-wide essential gene identification for SubAB toxin-induced cell death using CRISPR/CAS9 screening system

| PRJNA517089 | ENA

Genome-wide essential gene identification for Shiga toxin-induced cell death using CRISPR/CAS9 screening system with Vero cells

Project description:Genome-wide essential gene identification for Shiga toxin-induced cell death using CRISPR/CAS9 screening system with Vero cells

| PRJNA716329 | ENA

Identification of genes essential for the cell death induced by OSW-1 using the Genome-wide CRISPR-Cas9 knockout (GeCKO) screening

Project description:The Golgi stress response is an important cytoprotective system that enhances Golgi function in response to cellular demand, while cells damaged by prolonged Golgi stress undergo cell death. OSW-1, a natural compound with anticancer activity, potently inhibits OSBP that transports cholesterol and phosphatidylinositol-4-phosphate (PI4P) at contact sites between the endoplasmic reticulum and the Golgi apparatus. Previously, we reported that OSW-1 induces the Golgi stress response, resulting in Golgi stress- induced transcription and cell death. However, the underlying molecular mechanism has been unknown. To reveal the mechanism of a novel pathway of the Golgi stress response regulating transcriptional induction and cell death (the PI4P pathway), we performed a genome-wide knockout screen and found that transcriptional induction as well as cell death induced by OSW-1 was repressed by the loss of regulators of PI4P synthesis, such as PITPNB and PI4KB. Our data indicate that OSW-1 induces Golgi stress-dependent transcriptional induction and cell death through dysregulation of the PI4P metabolism in the Golgi.

2024-02-16 | GSE255970 | GEO

In vivo Genome-scale CRISPR-Cas9 knockout screening

Project description:To identify essential gene responding to anti-PD-1 immunotherapy, we performed in vivo Genome-scale CRISPR-Cas9 knockout screening. We found that cohesin complex invovled in regulation of anti-PD-1 immunotherapy.

2022-01-14 | GSE125201 | GEO

Cas9 induced cell death

Project description:Saturation guide RNA loading overcomes Cas9-induced human cell death

| PRJEB21268 | ENA

Gekko gecko

Project description:Gekko gecko overview

| PRJNA948070 | ENA

A CRISPR-based screen identifies genes essential for West Nile virus-induced cell death

Project description:West Nile virus (WNV) causes an acute neurological infection attended by massive neuronal cell death. However, the mechanism(s) behind the virus-induced cell death is poorly understood. Using a library containing 77,406 sgRNAs targeting 20,121 genes, we performed a genome-wide screen using CRISPR/Cas9. HEK 293FT cells were infected with lentivirus expressing sgRNAs and then transfected with a Cas9 expressing construct. WNV infection killed most cells during a 12d selection. Survivor cells were harvested, from which DNA was isolated. The sgRNAs integrated in genome of survivor cells were amplified with PCR. The PCR product was sequenced with Illumina MiSeq to profile the sgRNA population in the survivor cells. Three replicates were conducted. Similarly, a second round of screen was conducted. Among the genes identified, seven genes, EMC2, EMC3, SEL1L, DERL2, UBE2G2, UBE2J2, and HRD1, stood out as having the strongest phenotype, whose knockout conferred strong protection against WNV-induced cell death with two different WNV strains and in three cell lines. Interestingly, knockout of these genes did not block WNV replication. Thus, these appear to be essential genes that link WNV replication to downstream cell death pathway(s). In addition, the fact that all of these genes belong to the endoplasmic reticulum-associated protein degradation (ERAD) pathway suggests that this might be the primary driver of WNV-induced cell death. Examination of sgRNA populations in survival 293FT cells

2015-07-05 | E-GEOD-69666 | biostudies-arrayexpress

Genome-wide essential gene identification for lysenin-induced cell death using CRISPR/CAS9 screening system

Project description:Isolation of genes whose knockout conferring resistance to lysenin-Induced cell death

2023-04-30 | GSE185404 | GEO