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UHRF1 ubiquitin ligase activity supports the maintenance of low-density CpG methylation

ABSTRACT: UHRF1 ubiquitin ligase activity supports the maintenance of low-density CpG methylation

PROVIDER: PRJNA1077702 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28001731_1.fastq.gz	Fastqsanger.gz
	SRR28001731_2.fastq.gz	Fastqsanger.gz
	SRR28001732_1.fastq.gz	Fastqsanger.gz
	SRR28001732_2.fastq.gz	Fastqsanger.gz
	SRR28001733_1.fastq.gz	Fastqsanger.gz

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UHRF1 ubiquitin ligase activity supports the maintenance of low-density CpG methylation [BiSulfite-seq]

Project description:The E3 ubiquitin ligase UHRF1 is an established cofactor for replication-coupled DNA methylation inheritance. The current model posits a stepwise pathway in which UHRF1 engages nucleosomes through histone and DNA binding. This multivalent nucleosome readout directs UHRF1 ubiquitin ligase activity toward several lysines on N-terminal tails of histone H3, binding sites for DNMT1 through tandem ubiquitin interacting motifs (UIM1 and UIM2). While this elegant chromatin regulatory mechanism is now taking shape, the extent to which ubiquitin signaling through UHRF1 contributes to DNMT1-dependent DNA methylation maintenance genome-wide is not known. Here, we present comparative, genome-scale analysis of DNA methylation inheritance in human colon cancer cells with compromised UHRF1 ubiquitin ligase and DNMT1 ubiquitin reading activities. We reveal that DNA methylation maintenance in contexts of low CpG density is particularly vulnerable to disruption of UHRF1 ubiquitin ligase activity. This low CpG density hypomethylation signature, a common feature of partially methylated domains (PMDs) in human cancers, is also seen when DNMT1 ubiquitin reading activity through UIM1 is disrupted. Notably, disrupting UIM2 function affects DNA methylation inheritance to a similar extent as a catalytically dead form of the enzyme. Collectively, these studies demonstrate that DNMT1-dependent DNA methylation inheritance is a ubiquitin-regulated process and suggest that a disrupted UHRF1-DNMT1 ubiquitin signaling axis contributes to the development of PMDs in human cancers.

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UHRF1 ubiquitin ligase activity supports the maintenance of low-density CpG methylation [ChIP-seq]

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UHRF1 ubiquitin ligase activity supports the maintenance of low-density CpG methylation [EPIC methylation]

Project description:UHRF1 ubiquitin ligase activity supports the maintenance of low-density CpG methylation [EPIC methylation]

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UHRF1 ubiquitin ligase activity supports the maintenance of low-density CpG methylation [ChIP-seq]

Project description:UHRF1 ubiquitin ligase activity supports the maintenance of low-density CpG methylation [ChIP-seq]

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UHRF1 ubiquitin ligase activity supports the maintenance of low-density CpG methylation [BiSulfite-seq]

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Critical role of the UBL-domain in stimulating the E3 ubiquitin ligase activity of UHRF1 towards chromatin

Project description:The RING E3 ubiquitin ligase UHRF1 controls DNA methylation through its ability to target the maintenance DNA methyltransferase DNMT1 to newly replicated chromatin. DNMT1 recruitment relies on ubiquitylation of histone H3 by UHRF1, however, how UHRF1 deposits ubiquitin onto the histone is unknown. Here, we demonstrate that the ubiquitin-like domain (UBL) of UHRF1 is essential for RING-mediated H3 ubiquitylation. Using chemical crosslinking and mass spectrometry, biochemical assays and recombinant chromatin substrates we show that the UBL participates in structural rearrangements of UHRF1 upon binding to chromatin and the E2 ubiquitin conjugating enzyme UbcH5a/UBE2D1. Similar to ubiquitin, the UBL exerts its effects through a hydrophobic patch that contacts a regulatory surface on the “backside” of the E2 to stabilise the E2-E3-chromatin complex. Our analysis of the enzymatic mechanism of UHRF1 uncovers an unexpected function of the UBL-domain and defines a new role for this domain in DNMT1-dependent inheritance of DNA methylation.

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UHRF1 ubiquitin ligase activity supports the maintenance of low-density CpG methylation

Project description:This SuperSeries is composed of the SubSeries listed below.

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Critical role of the UBL-domain in stimulating the E3 ubiquitin ligase activity of UHRF1 towards chromatin

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