Project description:Rice roots grown in hydroponic culture were inoculated with rice blast fungus and gene expression profiles were analyzed by microarray Roots of two isogenic lines of rice cv Nipponbare (blast-resistance gene: Pia or pia) were inoculated with rice blast fungus, P91-15B, carrying avirulence gene, AvrPia. Total RNA was isolated from crown roots, labeled with cy3, and probed with agilent rice oligoarray (4x44).
Project description:In present study we compared transcriptional response to salinity stress between susceptible CO 12 and tolerant genotype trichy 1 of finger millet. We found out that several functional group of genes like transporters, transcription factors, genes involved in cell signalling, osmotic homeostasis, compatible solutes biosynthesis were upregulated more in tolerant genotype as compared to susceptible genotype in response to salinity stress. Salnity inhibited photosynthetic capacity and photosynthesis related genes more in susceptible genotype as compared to tolerant genotype. Identified genes are excellent targets for further functional studies in order to understand more specific molecular mechanisms of salt tolerance in two genotypes of finger millet and can be further used for developing salinity tolerant crops through genetic engineering.
Project description:Fourth leaves of rice seedlings (4.5 leaf stage) grown in hydroponic culture were inoculated with rice blast fungus and gene expression profiles were analyzed by microarray. Fourth leaves of the two isogenic lines of rice cv Nipponbare (blast-resistance gene: Pia or its mutant, pia) were inoculated with rice blast fungus, P91-15B, carrying avirulence gene, AvrPia. Total RNA was isolated, labeled with cy3, and probed with agilent rice oligoarray (4x44).
Project description:Fourth leaves of rice seedlings (4.5 leaf stage) grown in hydroponic culture were inoculated with rice blast fungus and gene expression profiles were analyzed by microarray. Fourth leaves of the two isogenic lines of rice cv Nipponbare (blast-resistance gene: Pish or its mutant, pish) were inoculated with rice blast fungus, Kyu77-07A, carrying avirulence gene, AvrPish. Total RNA was isolated, labeled with cy3, and probed with agilent rice oligoarray (4x44).
Project description:CRZ1 (Calcineurin Responsive Zinc finger 1) is a conserved transcription factor that relays Ca2+/calcineurin signals within the cell. To catalog genome wide locations that MoCRZ1 binds to, we have conducted chromatin immunoprecipitation coupled with microarray (ChIP-chip) in the rice blast fungus, Magnaporthe oryzae. Fungal mycelia were treated with 200mM CaCl2 to activate translocation of MoCRZ1 to the nucleus, thereby binding to the promoter regions of downstream genes, and CaCl2 together with 10ug/ml FK506 to block these phenomena. Using ChIP-chip, we were able to identify over 100 direct targets of MoCRZ1 which include well known downstream genes as well as previously unknown genes.
