Project description:To investigate the role of salicylic acid biosynthesis in transcriptional response to cytoskeleton disruption, we treated WT and sid2 plants (mutant deficient in ICS1) by latrunculin B for 24 h. We then performed gene expression profiling analysis using data obtained from RNA-seq of 3 biological replicates.
Project description:rs06-06_mirna - comparisons wt-mutants 2 - Time course : What are the genes (incuding microRNA precursors) that are differentially regulated upon flg22 treatment? miRNA : What are the genes (including miRNA precursors) that are differentially regulated in a set of microRNA mutants? siRNA : What are the genes (including miRNA precursors) that are differentially regulated in a set of siRNA mutants? - Ler, dcl1-9, hen1-1 and hasty were grown for 12-day on MS solid medium ,seedlings were then transferred in MS liquid medium and harvested 2 days after. Keywords: gene knock out
Project description:rs06-06_mirna - comparisons wt-mutants 1 - Time course : What are the genes (incuding microRNA precursors) that are differentially regulated upon flg22 treatment? miRNA : What are the genes (including miRNA precursors) that are differentially regulated in a set of microRNA mutants? siRNA : What are the genes (including miRNA precursors) that are differentially regulated in a set of siRNA mutants? - What are the genes (including miRNA precursors) that are differentially regulated in a set of tasiRNA mutants? Col-0, dcl4-1, rdr6-1 were grown for 12-day on MS solid medium ,seedlings were then transferred in MS liquid medium and harvested 2 days after. Keywords: gene knock out
Project description:The objective was to study the transcriptional profiles of fum null mutants vs the WT strain via RNA-seq, in order to find differentially expressed genes (DEGs, up or down regulated) in the WT strain in response to MMS treatment (0.35% [v/v],30 min), and more importantly in response to MMS+α-KG (0.35% [v/v], 30 min +25mM α-KG when indicated), compared to the mutant strains.
Project description:rs06-06_mirna - comparisons wt-mutants 1 et 3 - Time course : What are the genes (incuding microRNA precursors) that are differentially regulated upon flg22 treatment? miRNA : What are the genes (including miRNA precursors) that are differentially regulated in a set of microRNA mutants? siRNA : What are the genes (including miRNA precursors) that are differentially regulated in a set of siRNA mutants? - Col-0, dcl2-1, dcl3-1 and rdr2-1 were grown for 12-day on MS solid medium ,seedlings were then transferred in MS liquid medium and harvested 2 days after. Keywords: gene knock out
Project description:Transcriptional profiling of WT macrophages infected with WT L. monocytogenes for 180 minutes, with or without CAPE treatment Keywords: Drug comparison.
Project description:To explore the possible cause for the enhanced tolerance to osmotic stress exhibited by wrky54wrky70 double mutant, we have used Agilent Arabidopsis (V4) Gene Expression Microarray to characterize the effect of these mutations under the osmotic stress treatment. In addition to wild type and wrky54wrky70 double mutant, sid2-1 single and wrky54wrky70sid2-1 triple mutant were also included in the microarray experiment. When comparing to untreated control samples, 58 genes were identified as representatives to show the different expression level among different mutants under the 15% PEG6000 treatment for one day. Expression of six genes (RAB18, LTI78, KIN1,NCED3,P5CS1 and PRODH) from this gene list were quantified by qRT-PCR, confirming the suppressed induction in wrky54wrky70 double mutant under the osmotic stress. Osmotic stress induced gene expression in Col-WT, sid2-1 single, wrky54wrky70 double and wrky54wrky70sid2-1 triple mutants was measured after exposure to 15% PEG6000 treatment for one day. For each array, three labeled aRNA sample were hybridized and three biological replicates for each sample with dye swaps were made.
Project description:rs06-06_mirna - comparisons wt-mutants 2 - Time course : What are the genes (incuding microRNA precursors) that are differentially regulated upon flg22 treatment? miRNA : What are the genes (including miRNA precursors) that are differentially regulated in a set of microRNA mutants? siRNA : What are the genes (including miRNA precursors) that are differentially regulated in a set of siRNA mutants? - Ler, dcl1-9, hen1-1 and hasty were grown for 12-day on MS solid medium ,seedlings were then transferred in MS liquid medium and harvested 2 days after. Keywords: gene knock out 3 dye-swap - CATMA arrays
Project description:rs06-06_mirna - comparisons wt-mutants 1 - Time course : What are the genes (incuding microRNA precursors) that are differentially regulated upon flg22 treatment? miRNA : What are the genes (including miRNA precursors) that are differentially regulated in a set of microRNA mutants? siRNA : What are the genes (including miRNA precursors) that are differentially regulated in a set of siRNA mutants? - What are the genes (including miRNA precursors) that are differentially regulated in a set of tasiRNA mutants? Col-0, dcl4-1, rdr6-1 were grown for 12-day on MS solid medium ,seedlings were then transferred in MS liquid medium and harvested 2 days after. Keywords: gene knock out 2 dye-swap - CATMA arrays