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Analysis of a pair of HCA-7 derived cells with low expressing (CC) or high expressing (CC-CR) levels of miR-100 and miR-125b and RNA collected from Ago2 RNA immunoprecipitation

ABSTRACT: Analysis of a pair of HCA-7 derived cells with low expressing (CC) or high expressing (CC-CR) levels of miR-100 and miR-125b and RNA collected from Ago2 RNA immunoprecipitation

PROVIDER: PRJNA1081376 | ENA |

REPOSITORIES: ENA

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Analysis of a pair of HCA-7 derived cells with low expressing (CC) or high expressing (CC-CR) levels of miR-100 and miR-125b and RNA collected from Ago2 RNA immunoprecipitation (RNAseq_human_AGO2_IP.8635)

Project description:We report the results of RNA-Seq from a pair of HCA-7 derived cells with low, CC, and high, CC-CR, cells as well as RNA-seq from AGO2 RNA immunoprecipitation from the two cell lines in order to identify potential targets of miR-100 and miR-125b. Results were used to identify potential mRNAs that were upregulated in CC cells (low miR-100 and miR-125b), compared to CC-CR cells. In addition, we searched for potential mRNA targets that were enriched in the AGO2 immunoprecipitation of CC-CR cells.

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Project description:We report the results of RNA-Seq from an HCA-7 derived cell line with high levels of miR-100 and miR-125b (CC-CR) and knockout cell lines generated from the high expressing cells. CC-CR cells had miR-100, miR-125b or both miRNAs knocked out using CRISPR Cas9. Since miRNAs function to negatively regulate their target, the knockout cell lines should have the potential mRNA targets upregulated in comparison to CC-CR cells. Results were used to identify potential mRNAs that were upregulated in the knockout cell lines compared to CC-CR cells

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Analysis of an HCA-7 derived cell line (CC-CR) and cell lines generated with knockout of miR-100, miR-125b, or both miRNAs.

Project description:Analysis of an HCA-7 derived cell line (CC-CR) and cell lines generated with knockout of miR-100, miR-125b, or both miRNAs.

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RNA-sequencing analysis of 32Dclone3 cells overexpressing miR-125b

Project description:To better understand the mechanisms of blockage of myeloid differentiation and apoptosis and induction of proliferation by miR-125b, we preceded to identify miR-125b target genes involved in these pathways. We analyzed the total cellular gene expression pattern by RNA-sequencing of the parental 32Dclone3 myeloid cell line and that ectopically expressing miR-125b. We generated four cDNA libraries corresponding to duplicates of miR-125b and control cells. Compare the gene expression level in vector transduced 32Dclone3 cells with that in miR-125b transduced 32Dclone3 cells.

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RNA-sequencing analysis of 32Dclone3 cells overexpressing miR-125b

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