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Establishment of epithelial inflammatory injury model using adult kidney organoids [RNA-seq]

ABSTRACT: Establishment of epithelial inflammatory injury model using adult kidney organoids [RNA-seq]

PROVIDER: PRJNA1081385 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28122712_1.fastq.gz	Fastqsanger.gz
	SRR28122712_2.fastq.gz	Fastqsanger.gz
	SRR28122713_1.fastq.gz	Fastqsanger.gz
	SRR28122713_2.fastq.gz	Fastqsanger.gz
	SRR28122714_1.fastq.gz	Fastqsanger.gz

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Establishment of epithelial inflammatory injury model using adult kidney organoids [scRNA-seq]

Project description:Kidney organoids are emerging as an increasingly applied model system to hold great promise for transplantation to individuals with end-stage renal disease and as a useful tool for kidney research. Multiple iPSC-derived renal organoids have been established to understand kidney development and disease. However, few disease models originated from adult renal tissue with fully mature cell fates have been set up to recapitulate kidney inflammation or fibrosis. Here we created a novel organoid model that faithfully representing the cellular state of inflammatory response during the progression of renal disease upon TNFα exposure. scRNA-seq showed signatured inflammatory chemokines, cytokines and inflammation-associated signaling pathways were activated in TNFα-treatment organoids together with injury-associated markers such as LCN2 and CLU. Donor age is associated with TNFα-induced inflammatory reaction in organoids. Kidney organoids from young donors showed more transcriptional changes by down-regulation of TGFβ and extracellular matrix genes. In summary, we established an in vitro TNFα-treated kidney organoid model that representing the cellular state of the inflammatory response during renal disease progression and provided a novel tool for studying inflammation-related kidney disease and drug discovery.

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Establishment of epithelial inflammatory injury model using adult kidney organoids [RNA-seq]

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Establishment of epithelial inflammatory injury model using adult kidney organoids [scRNA-seq]

Project description:Establishment of epithelial inflammatory injury model using adult kidney organoids [scRNA-seq]

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Project description:Acute kidney injury (AKI) remains a major global healthcare problem and there is a need to develop human-based models to study AKI in vitro. Towards this goal, we have characterized induced pluripotent stem cell-derived human kidney organoids and their response to cisplatin, a chemotherapeutic drug that induces AKI and preferentially damages the proximal tubule. We found that a single treatment with 50 µM cisplatin induces HAVCR1 and CXCL8 expression, DNA damage (γH2AX) and cell death in the organoids in a dose-dependent manner but greatly impairs organoid viability. DNA damage was not specific to the proximal tubule but also affected the distal tubule and interstitial cell populations. This lack of specificity correlated with low expression of the proximal tubule-specific SLC22A2/OCT2 transporter for cisplatin. To improve viability, we developed a repeated low-dose regimen of 4x 5 µM cisplatin over 7 days and found this causing less toxicity while still inducing a robust AKI response that included secretion of known AKI biomarkers and inflammatory cytokines. This work validates the use of human kidney organoids to model aspects of AKI, with the potential to identify new AKI biomarkers and develop better therapies.

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