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MiRNAseq of extracellular vesicles from immortalized dental pulp mesenchymal stem cells from three different biopsies

ABSTRACT: miRNAseq of extracellular vesicles from immortalized dental pulp mesenchymal stem cells from three different biopsies

PROVIDER: PRJNA1081903 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28154476.fastq.gz	Fastqsanger.gz
	SRR28154477.fastq.gz	Fastqsanger.gz
	SRR28154478.fastq.gz	Fastqsanger.gz
	SRR28154479.fastq.gz	Fastqsanger.gz
	SRR28154480.fastq.gz	Fastqsanger.gz

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miRNAseq of extracellular vesicles from immortalized dental pulp mesenchymal stem cells from three different biopsies

Project description:Extracellular vesicles (EVs) derived from three different biopsies of dental pulp mesenchymal stem cells (MSC-EVs) were isolated and characterized by different omic analysis. The goal was to characterized the EVs cargo to study their implication in an animal model of chronic cardiac inflammation

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Project description:Human deciduous and permanent teeth exhibit different developmental processes, morphologies, histological characteristics and life cycles. In addition their pulp tissues react differently to external stimuli, such as the pulp sensitivity test, dental trauma and pulp therapy materials. These differences are attributable to their genetic backgrounds. Therefore the purpose of this study is to compare the differences of dental pulp in deciduous and permanent teeth. Pulp samples were obtained from permanent premolars (n=6, aged 11-14 years) and deciduous teeth (n=6, aged 11-14 years). Comparative cDNA microarrary analysis revealed several differences in gene expression between the deciduous and permanent pulp tissues. Each GSM record represents a pulp sample pooled from two teeth samples.

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Project description:Wnt regulates various cell responses. In dental pulp cells, Wnt signaling control cell proliferation, apoptosis, migration and differentiation. Here, the differential gene expression of human dental pulp stem cells treated with Wnt ligands or Wnt agonist was examined using a high throughput RNA sequencing technique. Results demonstrated that Wnt ligands or Wnt agonist altered numerous gene expression in human dental pulp stem cells.

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