Project description:Transcriptional profiling of Lactobacillus reuteri ATCC 55730 mid-log cultures before vs after exposure to 0.5% bovine bile (oxgall). Two sets of array experiments were performed. One set compared the expression profiles of L. reuteri ATCC 55730 cells before bile exposure vs cells that had been exposed to 0.5% bile for 15 minutes (bile shock). The other set compared the expression profiles of L. reuteri ATCC 55730 cells before bile exposure vs cells that had begun growing again in the presence of 0.5% bile (bile adaptation). Keywords: Stress response
Project description:Transcriptional profiling of Lactobacillus reuteri ATCC 55730 mid-log cultures before vs after exposure to 0.5% bovine bile (oxgall). Two sets of array experiments were performed. One set compared the expression profiles of L. reuteri ATCC 55730 cells before bile exposure vs cells that had been exposed to 0.5% bile for 15 minutes (bile shock). The other set compared the expression profiles of L. reuteri ATCC 55730 cells before bile exposure vs cells that had begun growing again in the presence of 0.5% bile (bile adaptation). Two sets of two condition experiments: Set one: before bile treatment and 15 minutes after bile treatment. Biological replicates: 5 untreated and 5 treated, independently grown and collected. Two replicates per biological replicate (dye-swap). Set two: before bile treatment and after the resumption of growth in the presence of bile. Biological replicates: 5 untreated and 5 treated, independently grown and collected. Two replicates per biological replicate (dye-swap).
Project description:Expression of stress responsive genes enables Limosilactobacillus reuteri to cross-protection against acid, bile salt and freeze-drying
