Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Engineered CRISPR-Cas12a for higher-order combinatorial chromatin perturbations (RNA-Seq)

ABSTRACT: Engineered CRISPR-Cas12a for higher-order combinatorial chromatin perturbations (RNA-Seq)

PROVIDER: PRJNA1083587 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR28217278_1.fastq.gz	Fastqsanger.gz
	SRR28217278_2.fastq.gz	Fastqsanger.gz
	SRR28217279_1.fastq.gz	Fastqsanger.gz
	SRR28217279_2.fastq.gz	Fastqsanger.gz
	SRR28217280_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 14

Similar Datasets

Engineered CRISPR-Cas12a for higher-order combinatorial chromatin perturbations

Project description:Engineered CRISPR-Cas12a for higher-order combinatorial chromatin perturbations

| PRJNA1083585 | ENA

Engineered CRISPR-Cas12a for higher-order combinatorial chromatin perturbations (Nanopore)

Project description:Engineered CRISPR-Cas12a for higher-order combinatorial chromatin perturbations (Nanopore)

| PRJNA1083588 | ENA

Engineered CRISPR-Cas12a for higher-order combinatorial chromatin perturbations (amplicon)

Project description:Engineered CRISPR-Cas12a for higher-order combinatorial chromatin perturbations (amplicon)

| PRJNA1083589 | ENA

Engineered CRISPR-Cas12a for higher-order combinatorial chromatin perturbations (CRISPR screen)

Project description:Engineered CRISPR-Cas12a for higher-order combinatorial chromatin perturbations (CRISPR screen)

| PRJNA1083590 | ENA

Engineered CRISPR-Cas12a for higher-order combinatorial chromatin perturbations (amplicon)

Project description:Multiplexed genetic perturbations are critical for testing functional interactions among coding or non-coding genetic elements. Compared to double-stranded DNA cutting, repressive chromatin formation using CRISPR interference (CRISPRi) avoids genotoxicity and is more effective for perturbing non-coding regulatory elements in pooled assays. However, current CRISPRi pooled screening approaches are limited to targeting 1-3 genomic sites per cell. We engineer an Acidaminococcus Cas12a (AsCas12a) variant, multiplexed transcriptional interference AsCas12a (multiAsCas12a), that incorporates R1226A, a mutation that stabilizes the ribonucleoprotein:DNA complex via DNA nicking. The multiAsCas12a-KRAB fusion improves CRISPRi activity over DNase-dead AsCas12a-KRAB fusions, often rescuing the activities of lentivirally delivered CRISPR RNAs (crRNA) that are inactive when used with the latter. multiAsCas12a-KRAB supports CRISPRi using 6-plex crRNA arrays in high-throughput pooled screens. Using multiAsCas12a-KRAB, we discover enhancer elements and dissect the combinatorial function of cis-regulatory elements in human cells. These results instantiate a group testing framework for efficiently surveying numerous combinations of chromatin perturbations for biological discovery and engineering.

2024-03-05 | GSE260830 | GEO

Engineered CRISPR-Cas12a for higher-order combinatorial chromatin perturbations (Nanopore)

2024-03-06 | GSE260827 | GEO

Engineered CRISPR-Cas12a for higher-order combinatorial chromatin perturbations (CRISPR screen)

2024-03-06 | GSE260829 | GEO

Engineered CRISPR-Cas12a for higher-order combinatorial chromatin perturbations (RNA-Seq)

2024-03-06 | GSE260828 | GEO

Optimized CRISPR-Cas12a platform for combinatorial drop-out genetic screening (RNA-seq)

Project description:Combinatorial genetic perturbations have been utilized to identify synthetic sick/lethal genetic interactions for cancer drug target discovery. Current methods for high-throughput combinatorial genetic screening are inefficient and cumbersome. Here we developed a simple, robust, and high-performance CRISPR-Cas12a-based approach for unbiased, combinatorial genetic screening in cancer cells.

2020-06-08 | GSE141130 | GEO

Engineered CRISPR-Cas12a for higher-order combinatorial chromatin perturbations

Project description:This SuperSeries is composed of the SubSeries listed below.

2024-03-05 | GSE260832 | GEO