Project description:The identification of natural and environmentally friendly pesticides is a key area of interest for the agrochemical industry, with many potentially active compounds being sourced from numerous plant species. In this study, we report the bioassay-guided isolation and identification of phytotoxic and antifungal compounds from the ethyl acetate extract of Helietta parvifolia stems. We identified eight compounds, consisting of two coumarins and six alkaloids. Among these, a new alkaloid, 2-hydroxy-3,6,7-trimethoxyquinoline-4-carbaldehyde (6), was elucidated, along with seven known compounds. The phytotoxicity of purified compounds was evaluated, and chalepin (4) was active against Agrostis stolonifera at 1 mM with 50% inhibition of seed germination and it reduced Lemna pausicotata (duckweed) growth by 50% (IC50) at 168 μM. Additionally, we evaluated the antifungal activity against the fungal plant pathogen Colletotrichum fragariae using a thin-layer chromatography bioautography assay, which revealed that three isolated furoquinoline alkaloids (flindersiamine (3), kokusagenine (7), and maculine (8)) among the isolated compounds had the strongest inhibitory effects on the growth of C. fragariae at all tested concentrations. Our results indicate that these active natural compounds, i.e., (3), (4), (7), and (8), could be scaffolds for the production of more active pesticides with better physicochemical properties.

| S-EPMC10708216 | biostudies-literature

The complete chloroplast genome sequence of Ulmus parvifolia (Ulmaceae).

Project description:Ulmus parvifolia Jacq is a kind of landscape tree endemic to East Asia. In this study, the complete chloroplast genome of U. parvifolia was sequenced. The genome was 159,259 bp in length, with a large single-copy (LSC) region of 88,451 bp, a small single-copy (SSC) region of 19,598 bp, and two inverted repeat (IR) regions of 25,605 bp, each. The genome consisted of 121 genes, including 77 protein-coding genes, 36 tRNA genes, and 8 rRNA genes. The phylogenetic results indicated that Ulmus is not monophyletic and U. parvifolia constitute a well-supported clade sister to U. Sect. Ulmus. The complete chloroplast genome of U. parvifolia will provide important information for phylogenetic and evolutionary studies in Ulmaceae, as well as the other closely related families.

| S-EPMC7782882 | biostudies-literature

Assembly of the Arabidopsis (Col-0) centromeres using Oxford Nanopore Technology long-read DNA sequencing.

Project description:Nanopore long-read sequencing was used to generate a complete assembly of the Arabidopsis centromeres.

2021-04-02 | E-MTAB-10272 | biostudies-arrayexpress

Comparison analysis of metabolite profiling in seeds and bark of Ulmus parvifolia, a Chinese medicine species

Project description:Ulmus parvifolia (U. parvifolia) was traditionally used in the treatment of some diseases such as inflammation, diarrhea and fevers. With the wide application of molecular biotechnology in plant development and utilization, the pharmaceutical value and chemical composition of the bark and leaves of U. parvifolia had been studied. However, metabolic signatures of seeds have not been studied. Seeds and bark of U. parvifolia were collected at the seeds ripening stage and metabolite profiling was performed through the untargeted metabolomics approach. A total of 2,578 and 2,207 metabolites were identified in seeds and bark, respectively. In particular, 574 differential metabolites were identified from the two parts of U. parvifolia, including flavonoids, terpene glycosides, triterpenoids and sesquiterpenoids. There are some bioactive compounds with antioxidant, anti-inflammatory and anti-cancer activities in these metabolites. More metabolites belonged to flavonoids and sesquiterpenoids were up-regulated in seeds than that in bark. There were more varieties of terpene glycosides and triterpenoids in bark than seeds. The pathway enrichment was performed, while flavonoid biosynthesis and flavone and flavonol biosynthesis were worthy of attention. Metabolic signatures of U. parvifolia seeds and bark were analyzed by untargeted metabolomics approach. Metabolite profiling, characteristics of differential metabolites and pathway enrichment were performed to evaluate the pharmaceutical value of seeds and bark. This study provided a scientific basis for the complementary use of the two different parts of U. parvifolia as a Chinese medicinal material.

2024-01-09 | MTBLS4572 | MetaboLights

Structural variation and DNA methylation shape the centromere-proximal meiotic crossover landscape in Arabidopsis

Project description:Nanopore Sequencing and assembly of Col-0 carrying seed coat expressed GFP and RFP transgenes flanking the centromere of chromosome 3 (CTL 3.9) - additionally, DNA methylation was derived using deepsignal-plant using these reads.

2024-01-20 | E-MTAB-13686 | biostudies-arrayexpress

Project description:Plastome and phylogenetic relationship of Torreya parvifolia

| PRJNA683944 | ENA