Project description:Ulmus parvifolia Jacq is a kind of landscape tree endemic to East Asia. In this study, the complete chloroplast genome of U. parvifolia was sequenced. The genome was 159,259 bp in length, with a large single-copy (LSC) region of 88,451 bp, a small single-copy (SSC) region of 19,598 bp, and two inverted repeat (IR) regions of 25,605 bp, each. The genome consisted of 121 genes, including 77 protein-coding genes, 36 tRNA genes, and 8 rRNA genes. The phylogenetic results indicated that Ulmus is not monophyletic and U. parvifolia constitute a well-supported clade sister to U. Sect. Ulmus. The complete chloroplast genome of U. parvifolia will provide important information for phylogenetic and evolutionary studies in Ulmaceae, as well as the other closely related families.
Project description:<p><em>Ulmus parvifolia</em> (<em>U. parvifolia</em>) was traditionally used in the treatment of some diseases such as inflammation, diarrhea and fevers. With the wide application of molecular biotechnology in plant development and utilization, the pharmaceutical value and chemical composition of the bark and leaves of <em>U. parvifolia</em> had been studied. However, metabolic signatures of seeds have not been studied. Seeds and bark of <em>U. parvifolia</em> were collected at the seeds ripening stage and metabolite profiling was performed through the untargeted metabolomics approach. A total of 2,578 and 2,207 metabolites were identified in seeds and bark, respectively. In particular, 574 differential metabolites were identified from the two parts of <em>U. parvifolia</em>, including flavonoids, terpene glycosides, triterpenoids and sesquiterpenoids. There are some bioactive compounds with antioxidant, anti-inflammatory and anti-cancer activities in these metabolites. More metabolites belonged to flavonoids and sesquiterpenoids were up-regulated in seeds than that in bark. There were more varieties of terpene glycosides and triterpenoids in bark than seeds. The pathway enrichment was performed, while flavonoid biosynthesis and flavone and flavonol biosynthesis were worthy of attention. Metabolic signatures of <em>U. parvifolia</em> seeds and bark were analyzed by untargeted metabolomics approach. Metabolite profiling, characteristics of differential metabolites and pathway enrichment were performed to evaluate the pharmaceutical value of seeds and bark. This study provided a scientific basis for the complementary use of the two different parts of <em>U. parvifolia</em> as a Chinese medicinal material.</p>
Project description:Nanopore Sequencing and assembly of Col-0 carrying seed coat expressed GFP and RFP transgenes flanking the centromere of chromosome 3 (CTL 3.9) - additionally, DNA methylation was derived using deepsignal-plant using these reads.
