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ABSTRACT: 16S rRNA of chicken cecum contents

PROVIDER: PRJNA1086343 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR28315700_1.fastq.gz	Fastqsanger.gz
	SRR28315700_2.fastq.gz	Fastqsanger.gz
	SRR28315800_1.fastq.gz	Fastqsanger.gz
	SRR28315800_2.fastq.gz	Fastqsanger.gz
	SRR28315701_1.fastq.gz	Fastqsanger.gz

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Metaproteomics of the Resistant Starch-fed Chronic Kidney Disease Rats

Project description:Background- Resistant starch is a prebiotic metabolized by the gut bacteria. It has been shown to attenuate chronic kidney disease (CKD) progression in rats. Previous studies employed taxonomic analysis using 16S rRNA sequencing and untargeted metabolomics profiling. Here we expand these studies by metaproteomics, gaining new insight into the host-microbiome interaction. Methods- Differences between cecum contents in CKD rats fed a diet containing resistant starch with those fed a diet containing digestible starch were examined by comparative metaproteomics analysis. Taxonomic information was obtained using unique protein sequences. Our methodology results in quantitative data covering both host and bacterial proteins. Results - 5,834 proteins were quantified, with 947 proteins originating from the host organism. Taxonomic information derived from metaproteomics data surpassed previous 16S RNA analysis, and reached species resolutions for moderately abundant taxonomic groups. In particular, the Ruminococcaceae family becomes well resolved – with butyrate producers and amylolytic species such as R. bromii clearly visible and significantly higher while fibrolytic species such as R. flavefaciens are significantly lower with resistant starch feeding. The observed changes in protein patterns are consistent with fiber-associated improvement in CKD phenotype. Several known host CKD-associated proteins and biomarkers of impaired kidney function were significantly reduced with resistant starch supplementation. Conclusions- Metaproteomics analysis of cecum contents of CKD rats with and without resistant starch supplementation reveals changes within gut microbiota at unprecedented resolution, providing both functional and taxonomic information. Proteins and organisms differentially abundant with RS supplementation point toward a shift from mucin degraders to butyrate producers.

2018-12-18 | PXD008845 | Pride

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Project description:16S rRNA of chicken cecal contents Raw sequence reads

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Project description:16s rRNA sequencing of chicken cecum at different laying stages

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