Project description:Flavonoid is an important secondary metabolite, which makes an important contribution to plant resistance to abiotic stress and human health. Fagopyrum tataricum (Tartary buckwheat) itself contains more flavonoids, so exploring the regulation of Tartary buckwheat flavonoid synthesis can provide a solid theoretical basis for the cultivation of new Tartary buckwheat varieties with high nutrition. In this paper, we studied the role of FtMYBs in regulating the biosynthesis of flavonoids in Tartary buckwheat.
Project description:We generated 61.9 Gb of high-quality sequencing data (~6.59 Gb per sample) and catalogued the expression profiles of 9,765 annotated Tartary buckwheat genes in each sample. The analysis showed differences of transcriptomes during the development of buckwheat. We identified numerous differentially expressed genes that exhibited distinct expression patterns. These genes have known or potential roles in development of buckwheat. Therefore, we are appealing candidates for further investigation of the gene expression and associated regulatory mechanisms.
Project description:We generated 76.61 Gb of high-quality sequencing data (~8.51 Gb per sample) and catalogued the expression profiles of 9,765 annotated Tartary buckwheat genes in each sample. The analysis showed differences of transcriptomes during the development of buckwheat seed. We identified numerous differentially expressed genes that exhibited distinct expression patterns. These genes have known or potential roles in development of buckwheat seed. Therefore, they are appealing candidates for further investigation of the gene expression and associated regulatory mechanisms related to development.
Project description:We generated 78.41 Gb of high-quality sequencing data (~8.71 Gb per sample) and catalogued the expression profiles of 9,765 annotated Tartary buckwheat genes in each sample. The analysis showed differences of transcriptomes during the development of easy dehulling buckwheat seed. We identified numerous differentially expressed genes that exhibited distinct expression patterns. These genes have known or potential roles in development of easy dehulling buckwheat seed. Therefore, we are appealing candidates for further investigation of the gene expression and associated regulatory mechanisms related to easy dehulling character.
Project description:High-fat diet (HFD) decreases insulin sensitivity. How high-fat diet causes insulin resistance is largely unknown. Here, we show that lean mice become insulin resistant after being administered exosomes isolated from the feces of obese mice fed a high-fat diet (HFD) or from human type II diabetic patients with diabetes. HFD altered the lipid composition of exosomes from predominantly PE in exosomes from lean animals (L-Exo) to PC in exosomes from obese animals (H-Exo). Mechanistically, we show that intestinal H-Exo is taken up by macrophages and hepatocytes, leading to inhibition of the insulin signaling pathway. Moreover, exosome-derived PC binds to and activates AhR, leading to inhibition of the expression of genes essential for activation of the insulin signaling pathway, including IRS-2, and its downstream genes PI3K and Akt. Together, our results reveal HFD-induced exosomes as potential contributors to the development of insulin resistance. Intestinal exosomes thus have potential as broad therapeutic targets.
