Project description:Monitoring microbial communities can aid in understanding the state of these habitats. Environmental DNA (eDNA) techniques provide efficient and comprehensive monitoring by capturing broader diversity. Besides structural profiling, eDNA methods allow the study of functional profiles, encompassing the genes within the microbial community. In this study, three methodologies were compared for functional profiling of microbial communities in estuarine and coastal sites in the Bay of Biscay. The methodologies included inference from 16S metabarcoding data using Tax4Fun, GeoChip microarrays, and shotgun metagenomics.
Project description:We are investigating the mRNA expression profiles of human lung cells to gaseous urban mixtures We used microarrays to compare the global mRNA expression profiles upon response to fresh against aged urban mix Keywords: dose A549 cells were grown to confluency and exposed to fresh urban mix, aged urban mix, or mock-treated. RNA was collected 9 hrs after exposure.
