Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effect of glycolysis on hepatic stellate cell transcriptome

ABSTRACT: Effect of glycolysis on hepatic stellate cell transcriptome

PROVIDER: PRJNA1088561 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR28361269_1.fastq.gz	Fastqsanger.gz
	SRR28361269_2.fastq.gz	Fastqsanger.gz
	SRR28361270_1.fastq.gz	Fastqsanger.gz
	SRR28361270_2.fastq.gz	Fastqsanger.gz
	SRR28361271_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 36

Similar Datasets

Effect of glycolysis on hepatic stellate cell transcriptome

Project description:To investigate the role of glycolysis in the regulation of HSC transcriptome, we treated our cells with or without glucose, to block or induce glycolysis, respectively. We then performed gene expression profiling analysis using data obtained from RNA-seq of all the conditions.

2024-03-23 | GSE261758 | GEO

Effect of SHP2 inhibition on hepatic stellate cell transcriptome.

Project description:Effect of SHP2 inhibition on hepatic stellate cell transcriptome.

| PRJNA588282 | ENA

Glycolysis in hepatic stellate cells coordinates fibrogenic extracellular vesicle release spatially to amplify liver fibrosis

Project description:Liver fibrosis is characterized by the activation of perivascular hepatic stellate cells (HSCs), the release of fibrogenic nano-sized extracellular vesicles (EVs) and increased HSC glycolysis. Nevertheless, how glycolysis in HSCs coordinates fibrosis amplification through tissue zone-specific pathways remains elusive. Here, we demonstrate that HSC-specific genetic inhibition of glycolysis reduced liver fibrosis. Moreover, spatial transcriptomics revealed a fibrosis-mediated upregulation of EV-related pathways in the liver pericentral zone, which was abrogated by the glycolysis genetic inhibition. Mechanistically, glycolysis in HSCs upregulated the expression of EV-related genes such as RAB31 by enhancing histone-3-lysine-9 acetylation on the promoter region, which increased EV release. Functionally, these glycolysis-dependent EVs increased fibrotic gene expression in recipient HSC. Furthermore, EVs derived from glycolysis-deficient mice abrogated liver fibrosis amplification in contrast to glycolysis-competent mouse EVs. In summary, glycolysis in HSCs amplifies liver fibrosis by promoting fibrogenic EV release in the hepatic pericentral zone, which represents a potential therapeutic target.

2024-05-16 | PXD050337 | Pride

Effect of SHP2 inhibition on hepatic stellate cell transcriptome.

Project description:Hepatic stellate cell autophagy inhibits extracellular vesicle release to attenuate liver fibrosis. Primary human hepatic stellate cells were treated with PDGF or PDGF + SHP2 inhibitor. RNA was purified and submitted for sequencing to Mayo Clinic Genomics Core. After applying the filters FDR>0.05, Log2(FC)>1 and RPKM>15, we ended up with nearly 300 genes differentially regulated between the two conditions. Ingenuity Pathway Analysis revealed that Ostheoarthritis was the first pathway to be differentially regulated. From this pathway, REDD1 (DDIT4 transcript), an mTOR inhibitor, was further explored, especially in the context of extracellular vesicle release.

2019-11-08 | GSE140110 | GEO

Recombinant human GDF2 protein effect on human hepatic stellate cell line LX2

Project description:Analysis of human hepatic stellate cell line LX2 stimulated for 24h in serum-free DMEM medium containing 0 or 50 ng/ml recombinant human GDF2 protein. Results provide insight into the activation effects of GDF2 on human hepatic stellate cell. We used microarrays to detail the global programme of gene expression underlying activation of hepatic stellate cells and identified liver-fibrosis-related genes genes during this process.

2019-09-05 | GSE104482 | GEO

Effect of TET2 deficient immune cells on hepatic stellate cell activation in vitro [coculture]

Project description:To investigate the role of Tet2 deficient immune cells in hepatic stellate cell activation, wild type or Tet2 deficient B cells, T cells, and hepatic macrophages were isolated and co-cultured with purified hepatic stellate cells. Gene expression profiling analysis of bulk hepatic stellate cell RNA was then performed.

2023-02-07 | GSE223693 | GEO

TLR4 or CD44 deficiency effect on hepatic stellate cells

Project description:Analysis of hepatic stellate cells isoltaed from wild-type, TLR4-/-, and CD44-/- mice. TLR4 and CD44 are major hyaluronic acid receptors. Results provide insight into the effects of TLR4 and CD44 loss in hepatic stellate cells.

2020-01-01 | GSE109200 | GEO

Inhibition of Enhancer of Zeste Homologue 2 attenuates TGF-β dependent hepatic stellate cell activation and liver fibrosis

Project description:We report the effect of TGFβ vs PDGF 2h treatment in hepatic stellate cells. We also report the effect of TGFβ treatment for 48h in human hepatic stellate cells.

2018-09-07 | GSE119606 | GEO

TLR4 or CD44 deficiency effect on hepatic stellate cells

Project description:TLR4 or CD44 deficiency effect on hepatic stellate cells

| PRJNA430098 | ENA

Hepatic Stellate Cell Activation after 72hr

Project description:Hepatic Stellate Cell Activation after 72hr

| PRJNA948023 | ENA