Project description:This SuperSeries is composed of the following subset Series:; GSE10726: Expression data from skin of epithelial activated beta-catenin mutant mouse embryo; GSE10727: Expression data from dermis of epithelial activated beta-catenin mutant mouse embryo; GSE10728: Expression data from epidermis of epithelial activated beta-catenin mutant mouse embryo Experiment Overall Design: Refer to individual Series
Project description:Forced expression of activated beta-catenin in mouse dermal fibroblasts is sufficient to cause spontaneous, progressive skin fibrosis in vivo. We generated triple-transgenic HoxB6CreERT/+; R26-YFP/+; CatnbΔex3/+ "activated beta-catenin" mice and double-transgenic HoxB6CreERT/+; R26-YFP/+ littermate control mice. We induced Cre activity (resulting in expression of activated beta-catenin in triple-transgenic mutant fetuses) by administering tamoxifen to the pregnant dam at embryonic day 16.5. The activated beta-catenin mice developed fibrotic skin, characterized by elevated collagen deposition and increased fibroblast proliferation. We performed RNA-sequencing to profile gene expression in the dermis of control and activated beta-catenin mutant mice with established skin fibrosis at 3 weeks of age.
Project description:β-catenin signaling is required for hair follicle development, but it is unknown whether it is sufficient to activate expression of hair follicle genes in embryonic skin. To address this we profiled gene expression in skin dissected from E14.5 KRT14-Cre Ctnnb1(Ex3)fl/+ embryos carrying an activating mutation in epithelial beta-catenin, and control littermate embryos. Experiment Overall Design: Total skin RNA from two KRT14-Cre Ctnnb1(Ex3)fl/+ and two control littermate E14.5 embryos was hybridized to Affymetrix GeneChip Mouse Genome MOE430 2.0 oligonucleotide microarrays. Experiment Overall Design: Appended below is Table S3: Full list of differentially expressed genes in KRT14-Cre Ctnnb1(Ex3)fl/+ mutant compared with control littermate intact skin at E14.5, including normalization and filter parameters. Fold change, listed in the second column, gives the ratio of normalized mutant : control transcript levels.
Project description:The Wnt/β-catenin signaling pathway is crucial for the development of variety of organs including the mammary gland. However, the precise role of Wnt/β-catenin signaling during embryonic mammary gland morphogenesis is still poorly understood. Here, we used an epithelial gain-of-function β-catenin mouse model to study the role of Wnt/β-catenin signaling in embryonic mammary gland development and profiled the transcriptomes of E13.5 and E16.5 control and mutant mammary epithelia.
