Lupus neutrophils transcriptome
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1089226 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR28380564_1.fastq.gz | Fastqsanger.gz | |||
| SRR28380564_2.fastq.gz | Fastqsanger.gz | |||
| SRR28380565_1.fastq.gz | Fastqsanger.gz | |||
| SRR28380565_2.fastq.gz | Fastqsanger.gz | |||
| SRR28380566_1.fastq.gz | Fastqsanger.gz |
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Project description:Our group has proposed that low-density granulocytes (LDGs) play an important role in lupus pathogenesis, as they can damage endothelial cells and synthesize increased levels of proinflammatory cytokines and type I interferons. LDGs have a heightened capacity to synthesize neutrophil extracellular traps (NETs). NETs from LDGs display increased levels of bactericidal and immunostimulatory proteins, such as the cathelicidin LL37 and externalize double-stranded DNA (dsDNA). Lupus netting LDGs have increased capacity to kill endothelial cells and expose IL-17. Through NETosis, lupus neutrophils stimulate plasmacytoid DCs to synthesize IFN-α. Our results further expand the potential pathogenic role of aberrant lupus neutrophils through a NET-mediated effect. We used microarrays to analyze the gene expression of neutrophils in healthy and lupus patients, and of low-density granulocytes in lupus patients.
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