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ABSTRACT:
PROVIDER: PRJNA1089530 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:In this study, roots of Rehmannia glutinosa were used as experimental material, and three tuber roots of Rehmannia glutinosa in extension stage (I), expansion stage (E) and mature stage (M) were selected as samples, The iTRAQ quantitative proteomic technology combined with two-dimensional liquid chromatography and tandem mass spectrometry (2D-LC-MSMS) technology was used to construct the root tuber proteome database of Rehmannia glutinosa, the genes related to the growth and development of Rehmannia glutinosa root tuber were found.
2022-02-17 | PXD025914 | Pride
Project description:Although processed potato tuber texture is an important trait that influences consumer preference, a detailed understanding of tuber textural properties at the molecular level is lacking. Previous work has identified tuber pectin methyl esterase activity (PME) as a potential factor impacting on textural properties and the expression of a gene encoding an isoform of PME (PEST1) was associated with cooked tuber textural properties. In this study a transgenic approach was undertaken to investigate further the impact of the PEST1 gene. Antisense and over-expressing potato lines were generated. In over-expressing lines tuber PME activity was enhanced by up to 2.3 fold whereas in antisense lines PME activity was decreased by up to 38%. PME isoform analysis indicated that the PEST1 gene encoded one isoform of PME. Analysis of cell walls from tubers from the over-expressing lines indicated that the changes in PME activity resulted in a decrease in pectin methylation. Analysis of processed tuber texture demonstrated that the reduced level of pectin methylation in the over-expressing transgenic lines was associated with a firmer processed texture. Thus there is a clear link between PME activity, pectin methylation and processed tuber textural properties.
2010-12-01 | E-TABM-1062 | biostudies-arrayexpress
Project description:Transcriptional analysis of pigmented and non-pigmented potato tuber flesh using POCI 44k microarrays
2010-01-01 | E-TABM-787 | biostudies-arrayexpress
Project description:Transcriptional analysis of pigmented and non-pigmented potato tuber flesh using POCI 44k microarrays
2010-01-01 | E-TABM-788 | biostudies-arrayexpress
Project description:The aim of this analysis was to better understand the complex symbiotic stage of Tuber melanosporum by combining the use of laser capture microdissection and microarray gene expression analysis. We isolated the fungal/soil (i.e. the mantle) and the fungal/plant (i.e. the Hartig net) interfaces from transverse sections of T. melanosporum/Corylus avellana ectomycorrhizas and identified the transcriptional landscape associated with each compartment. We compared these data to the transcriptome of ectomycorrhizal root tips, free-living mycelium and fruiting bodies of Tuber melanosporum (Series GSE17529).
2013-03-01 | GSE36870 | GEO
Project description:Nitrogen (N) can be absorbed by plants, thereby affects plant physiological activity, interferes gene expression, alters metabolite content and influences plant growth. However, the molecular mechanism underlying the potato tuberization response to nitrogen remains unclear. The plants were cultivated in the pots using N-deficient, N-Routine and N-sufficient conditions. Physiological response analysis, transcriptomics and metabolomics were performed on potato stolon exposed to Nitrogen stress. Transcriptomics analysis revealed that 2756 differentially expressed genes (DEGs) responded to nitrogen stress. By using metabolomics analysis, a total of 600 d differentially accumulated metabolites (DAMs) were identified. Further correlation analysis of major DEGs and DAMs showed that 9 key DEGs were involved in alpha-linolenic acid metabolism, 16 key DEGs in starch and sucrose metabolism, 7 key DEGs in nitrogen metabolism, and 16 key DEGs in ABC transporters. Nitrogen deficiency significantly up-regulated the contents of sucrose, GDP-glucose and L-glutamic acid, and promoted the growth of stolon by up-regulating the expression of AMY, SBE, SS, SPS, AGPS and NR-related genes. However, High nitrogen is the opposite. In addition, high nitrogen treatment up-regulated EG, SUS and GDH related genes, accumulated a large number of 9 (S) -HpOTr E, 13 (S) -HpOTr E and L-Glutamine, ultimately affected the balance between plant growth and defense. In general, our study revealed the co-expressed genes and potential pathways related to potato tuber formation under different nitrogen conditions. These comprehensive analysis data provide a better understanding of improving potato tuber traits at the molecular and metabolic levels.
2024-10-23 | GSE271112 | GEO