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High-throughout sequencing of ovarian cancer cells treated with TMPyP4 and H2O2

ABSTRACT: High-throughout sequencing of ovarian cancer cells treated with TMPyP4 and H2O2

PROVIDER: PRJNA1090047 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28398000_1.fastq.gz	Fastqsanger.gz
	SRR28398000_2.fastq.gz	Fastqsanger.gz
	SRR28398001_1.fastq.gz	Fastqsanger.gz
	SRR28398001_2.fastq.gz	Fastqsanger.gz
	SRR28397996_1.fastq.gz	Fastqsanger.gz

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High-throughout sequencing of ovarian cancer cells treated with TMPyP4 and H2O2

Project description:The high-throughput sequencing technology was performed after the treatment of human ovarian cancer cells A2780 with TMPyP4 and H2O2 purchased from Sigma-Aldrich, to explore the expression of genes related to cell proliferation, DNA damage and ROS levels of ovarian cancer cells A2780 after the treatment of TMPyP4 and H2O2, and to find an interesting target pathway,HIF-1 signaling pathway.

2024-03-25 | GSE262015 | GEO

High-throughout sequencing of ovarian cancer cells treated with Annonacin and Carboplation

Project description:High-throughout sequencing of ovarian cancer cells treated with Annonacin and Carboplation

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H2O2-treated and untreated HCT116 cells

Project description:H2O2-treated and untreated HCT116 cells

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RNA sequencing of hydrogen peroxide (H2O2)-treated melanocytes

Project description:Purpose: Determine the mechanism of H2O2-induced signaling in melanocytes. Method: Primary human epidermal melanocytes were treated with H2O2 (200 μM) and incubated for 24 h. Total RNA (500 ng) from melanocytes were extracted and subjected to library synthesis. Results: H2O2-treated melanocytes exhibited upregulation of cell death and type 1 interferon-related genes. Conclusion: H2O2-induced melanocyte signaling was well evaluated using RNA sequencing.

2023-07-28 | GSE216447 | GEO

High-throughout sequencing of ovarian cancer cells treated with Annonacin and Carboplation

Project description:The high-throughput sequencing technology was performed after the treatment of human ovarian cancer cells A2780 and ES2 with Annonacin and Carboplation purchased from MCE, to explore the expression of genes related to cell proliferationand DNA damage of ovarian cancer cells A2780 and ES2 after the treatment of Annonacin and Carboplation, and to find an interesting target pathway,p53 signaling pathway.

2024-12-14 | GSE283821 | GEO

Human SW480 cells: Untreated vs H2O2 Treated

Project description:ChIP-on-chip tilling array comparing untreated human SW480 cells vs SW480 cells treated with 2mM H2O2 for 30min. Exposing cells to the reactive oxygen species, hydrogen peroxide, recruits DNA methyltransferase 1 (DNMT1) to damaged chromatin. DNMT1 becomes part of a complex(es) containing DNMT3B and members of Polycomb Repressive Complex 4. The goal was to determine the effect of hydrogen peroxide treatment on chromatin, including changes in histone modifications and binding patterns of chromatin-associated proteins. [Agilent-014706]: Two-condition: untreated SW480 cells (U) vs H2O2 treated SW480 cells (T). Five-mark: SIRT1, gamma-HA2X, DNMT1, DNMT3B, and EZH2. [Agilent-014707]: Two-condition: untreated SW480 cells (U) vs H2O2 treated SW480 cells (T). Five-mark: SIRT1, gamma-HA2X, DNMT1, DNMT3B, and EZH2. [Agilent-026595]: Two-condition: untreated SW480 cells (U) vs H2O2 treated SW480 cells (T). Five-mark: SIRT1, gamma-HA2X, DNMT1, DNMT3B, and H3. [Agilent-027811]: Two-condition: untreated SW480 cells (U) vs H2O2 treated SW480 cells (T). Four-mark: H3, 3MeK4H3, AcK16H4, and 3MeK27H3.

2011-12-01 | E-GEOD-32382 | biostudies-arrayexpress

High-throughout sequencing of liver cancer cells treated Flavokawain C

Project description:High-throughout sequencing of liver cancer cells treated Flavokawain C

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RNA sequencing of hydrogen peroxide (H2O2)-treated melanocytes

Project description:RNA sequencing of hydrogen peroxide (H2O2)-treated melanocytes

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High-throughout sequencing of RAW264.7 cells treated with LPS and Harmine

Project description:High-throughout sequencing of RAW264.7 cells treated with LPS and Harmine

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Expression profiling of small RNAs in control and H2O2-treated rice seedlings

Project description:We aimed to identify putative H2O2-regulated miRNAs expressed in rice seedlings under oxidative stress by using a deep sequencing approach developed by Solexa (Illumina). Two small RNA libraries were constructed from H2O2-treated and control rice seedlings, and more than five million small RNA sequence reads were generated for each library. We identified seven H2O2-responsive miRNA families via expression profiling of the miRNAs based on a comparative miRNAomic analysis in combination with experimental validation. Furthermore, 32 miRNAs, 17 from known miRNA loci and 15 from novel miRNA loci, were also newly identified from the sequencing data. To the best of our knowledge, this is the first report of a systematic investigation of H2O2-regulated miRNAs and their targets in plants.

2010-08-15 | GSE23217 | GEO

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