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MEG3 Chromatin isolation by RNA purification-DNA sequencing (ChIRP-Seq) in wild type Mouse emryonic fibroblasts (MEFs) and Beta actin Knock out MEFS

ABSTRACT: MEG3 Chromatin isolation by RNA purification-DNA sequencing (ChIRP-Seq) in wild type Mouse emryonic fibroblasts (MEFs) and Beta actin Knock out MEFS

PROVIDER: PRJNA1090412 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28407700_1.fastq.gz	Fastqsanger.gz
	SRR28407700_2.fastq.gz	Fastqsanger.gz
	SRR28407701_1.fastq.gz	Fastqsanger.gz
	SRR28407701_2.fastq.gz	Fastqsanger.gz
	SRR28407702_1.fastq.gz	Fastqsanger.gz

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MEG3 Chromatin isolation by RNA purification-DNA sequencing (ChIRP-Seq) in wild type Mouse emryonic fibroblasts (MEFs) and Beta actin Knock out MEFS

Project description:In the eukaryotic cell, β-actin plays essential nuclear functions. Actin directly affects genome organization and regulates promoter-enhancer interactions as part of the BAF (SWI/SNF) chromatin remodeling complex. It also binds to the hyperphosphorylated carboxy-terminal domain (CTD) of RNA polymerases II, and depletion of β-actin leads to differential gene expression that impacts key cellular processes such as differentiation. In this study, we investigated whether β-actin controls the level of long non-coding RNAs (lncRNAs). Using a combination of bulk RNA-seq and qPCR analyses on total RNA isolated from WT mouse embryonic fibroblasts (MEFs), β-actin heterozygous (HET) MEFs, and β-actin KO MEFs. Our findings demonstrate that the expression of several lncRNAs is directly affected by β-actin depletion. One of these lncRNAs, Meg3, shows increased expression in a dosage-dependent manner. Using ChIRP-seq, we observed alterations in Meg3 genomic association and Meg3 enrichment at or near enhancer sites following β-actin depletion. These changes were accompanied by increased levels of H3K27 acetylation. At these sites, specific Meg3 enrichment disrupts promoter-enhancer interactions, as revealed by the Activity by Contact (ABC) model, through a sponging effect. Consequently, this leads to the repression of genes involved in metabolic biosynthetic pathways for chondroitin, heparan, dermatan sulfate, and phospholipases, impacting their synthesis. We propose that Meg3 potentially impairs local genome organization (or DNA looping) and negatively affects gene expression through its chromatin sponging effect that interferes with promoter-enhancer interactions.

2025-03-28 | GSE262113 | GEO

Long Non-Coding RNA MEG3 Promotes Angiogenesis by directly regulating integrin signaling impairing endothelial cell function [ChIRP-seq]

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RNA-Seq analysis of beta-actin knockout MEFs expressing NLS-tagged beta-actin

Project description:RNA-Seq analysis of beta-actin knockout MEFs expressing NLS-tagged beta-actin to study role of actin in transcription

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Sequencing analysis of beta-actin KO MEFs expressing NLS-beta-actin

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Meg3 binding sites in mouse insulinoma cells

Project description:We have shown that increased β-cell proliferation in functioning pancreatic neuroendocrine tumors (insulinomas) correlated with reduced expression of the long non-coding RNA Meg3 and increased expression of the oncogenic receptor c-Met. To investigate the target binding sites of Meg3 in and around the c-Met gene, we did ChIRP-Seq using biotinylated probes from the mouse Meg3 RNA sequence. This would help us better understand how Meg3 regulates ithe expression of c-Met to control β-cell proliferation in insulinoma cells.

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RNA-Seq analysis of beta-actin knockout MEFs expressing NLS-tagged beta-actin

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