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Biochemical-free enrichment or depletion of RNA classes in real-time during direct RNA sequencing with RISER (cell lines)

ABSTRACT: Biochemical-free enrichment or depletion of RNA classes in real-time during direct RNA sequencing with RISER (cell lines)

PROVIDER: PRJNA1090960 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28796306_1.fastq.gz	Fastqsanger.gz
	SRR28796307_1.fastq.gz	Fastqsanger.gz
	SRR28796308_1.fastq.gz	Fastqsanger.gz
	SRR28796309_1.fastq.gz	Fastqsanger.gz
	SRR28796310_1.fastq.gz	Fastqsanger.gz

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Biochemical-free enrichment or depletion of RNA classes in real-time during direct RNA sequencing with RISER (cell lines)

Project description:The heterogeneous composition of cellular transcriptomes poses a major challenge for detecting weakly expressed RNA classes, as they can be obscured by abundant RNAs. Although biochemical protocols can enrich or deplete specified RNAs, they are time-consuming, expensive and can compromise RNA integrity. Here we introduce RISER, a biochemical-free technology for the real-time enrichment or depletion of RNA classes. RISER performs selective rejection of molecules during direct RNA sequencing by identifying RNA classes directly from nanopore signals with deep learning and communicating with the sequencing hardware in real time. By targeting the dominant messenger and mitochondrial RNA classes for depletion, RISER reduced their respective read counts by more than 85%, resulting in an increase in sequencing depth of up to 93% for long non-coding RNAs. We also applied RISER for the depletion of globin mRNA in whole blood, achieving a decrease in globin reads by more than 90% as well as a significant increase in non-globin reads. Furthermore, using a GPU or a CPU, RISER is faster than GPU-accelerated basecalling and mapping. RISER’s modular and retrainable software and intuitive command-line interface allow easy adaptation to other RNA classes. RISER is available at https://github.com/comprna/riser.

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Biochemical-free enrichment or depletion of RNA classes in real-time during direct RNA sequencing with RISER

Project description:Biochemical-free enrichment or depletion of RNA classes in real-time during direct RNA sequencing with RISER

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Biochemical-free enrichment or depletion of RNA classes in real-time during direct RNA sequencing with RISER (whole blood)

Project description:Biochemical-free enrichment or depletion of RNA classes in real-time during direct RNA sequencing with RISER (whole blood)

| PRJNA1090961 | ENA

Biochemical-free enrichment or depletion of RNA classes in real-time during direct RNA sequencing with RISER

Project description:This SuperSeries is composed of the SubSeries listed below.

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Biochemical partitioning of RNA collectives reveals ProQ as a global RNA chaperone in bacteria

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| S-EPMC390331 | biostudies-literature

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