Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: BSR-seq for mapping the causal gene responsible for the formation of prickles in eggplant

PROVIDER: PRJNA1091114 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR28426265_1.fastq.gz	Fastqsanger.gz
	SRR28426265_2.fastq.gz	Fastqsanger.gz
	SRR28426266_1.fastq.gz	Fastqsanger.gz
	SRR28426266_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 4 of 4

Similar Datasets

Project description:BSR-seq for mapping the causal gene responsible for the green intensity in lettuce leaves

| PRJNA731301 | ENA

Gene Mapping via Bulked Segregant RNA-Seq (BSR-Seq)

Project description:Bulked segregant analysis (BSA) is an efficient method to rapidly and efficiently map genes responsible for mutant phenotypes. This procedure, however, requires access to quantitative genetic markers that are polymorphic in the mapping population. We have developed a modification of BSA (BSR-Seq) that makes use of RNA-Seq reads to efficiently map genes even in populations for which no polymorphic markers have been previously identified. Because of the digital nature of next-generation sequencing (NGS) data, it is possible to conduct de novo SNP discovery and quantitatively genotype BSA samples using the same RNA-Seq data. In addition, analysis of the RNA-Seq data provides information on the effects of the mutant on global patterns of gene expression at no extra cost. In combination these results greatly simplify gene cloning experiments. To demonstrate the utility of this strategy BSR-Seq was used to clone the glossy3 (gl3) gene of maize. Mutants of the glossy loci exhibit altered accumulation of epicuticular waxes on juvenile leaves. We previously generated a large collection of glossy mutants using the Mu transposon system. By subjected a reference allele to BSR-Seq, we were able to map the gl3 locus to a ~2.3Mb interval that is consistent with the results of prior mapping experiments. The single gene located in the 2.3Mb mapping interval that contained a Mu insertion and whose expression was down-regulated in the mutant pool was subsequently demonstrated to be the gl3 gene via the analysis of multiple independently Mu transposon induced mutant alleles. The gl3 gene encodes a putative myb transcription factor, which directly or indirectly affects the expression of a number of genes involved in the biosynthesis of very-long-chain fatty acids.

2012-12-03 | E-MTAB-4234 | biostudies-arrayexpress

Project description:BSR-seq for mapping a sweet gene in maize

| PRJNA770099 | ENA

Project description:Preliminary mapping of Brcwm by BSR-seq.

| PRJNA919407 | ENA

Project description:Primary mapping of the BoVl gene via BSR-seq analysis

| PRJNA900045 | ENA

Project description:BSR-seq for mapping maize kernel cadmium content-related gene

| PRJNA656212 | ENA

Project description:BSR-seq for mapping southern corn rust-resistant gene in maize

| PRJNA680211 | ENA

iTRAQ-based quantitative proteomics of eggplant root treated with waterlogging stress

Project description:Cultivated eggplant XN, a waterlogging-tolerant variety, were treated with waterlogging stress, and the root of XN eggplant were harvested at the time point of 0, 6, 12, and 24 h post treatment ,relatively. iTRAQ-based quantitative proteomics was performed to analyze protein dynamics in eggplant root.

2023-07-20 | PXD041747 | Pride

Project description:Causal gene mapping for aam1 by MutMap

| PRJNA1055747 | ENA

Transcriptome analysis of Eggplant (Solanum melongena L.) cv. PPL during fruit development and ripening.

Project description:Transcriptome analysis of Eggplant cv. PPL during fruit development at 0, 5, 10, 20 and 50 dpa. Eggplant is third most important solanaceae crop species after potato and tomato. It is a versatile crop adapted to different agro-climatic regions and can be grown throughout the year. Unripe eggplant fruit is consumed as cooked vegetable in various ways. It is low in calories and fats, contains mostly water, some protein, fibre and carbohydrates. To decipher molecular mechanisms involved in fruit development eggplant fruit were collected at 0, 5, 10, 20 and 50 dpa and gene expression profiles were analyzed using Affymetrix tomato GeneChip Genome array.

2014-02-14 | GSE44182 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data