Drosophila melanogaster
Ontology highlight
ABSTRACT:
OTHER RELATED OMICS DATASETS IN: GSE11479
PROVIDER: PRJNA109117 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Ectopic activation of the immune response in the larval airway epithelium
| PRJNA109115 | ENA
Project description:The unsurpassed simplicity of the fruitflyâs airway epithelium, that is made of homogenous epithelial cells only, favours its use as a model to study general features and response characteristics of airway epithelia in general. All epithelial cells are able to launch an immune response as characterized by the expression of antimicrobial peptide genes. Infection induces a complex change in the expression profile of these epithelial cells. Outstanding are a priming of the immune system and the launch of a survival program, presumably to counteract infection induced apoptotic signals, which comprises the concurrent expression of known longevity genes such as dFoxo, and dThor. In regions of the airway epithelium with strong immune reactions, a complex remodelling of the airways can be observed, which is characterized by metaplasia and presumably also by hyperplasia of the affected epithelial cells. At the transcriptional level, this reorganization of the airway epithelium is mirrored by a recapitulation of genetic programs that are characteristic for early phases of airway development. Taken together, the response characteristics of the flyâs airway epithelium towards infections discloses features that are known from inflammatory diseases of the human lung, thus opening the opportunity to study fundamental aspects of these diseases in the fly. Keywords:infection, Erwinia c., third instar larva, airway epithelium, two-colour microarray Infection of the airway epithelium by the gram-negativ bacteria Erwinia carotovora. For the infection experiments third instar larvae of the GFP-reporter strain YW DD1 were used and only isolated when the whole epithelium of the airway epithelium showed GFP expression. Uninfected larvae were used as controls. In general, four replicates were performed including dye-swaps. A table of significantly-regulated genes from the SAM-output and GeneTraffic-output has been linked as a supplementary file at the foot of this record.
2010-05-19 | E-GEOD-11444 | biostudies-arrayexpress
Project description:Larval airway epithelium response to infection
| PRJNA106403 | ENA
Project description:Transcriptional regionalization of the fruit fly's airway epithelium
| PRJNA231519 | ENA
Project description:The unsurpassed simplicity of the fruitfly’s airway epithelium, that is made of homogenous epithelial cells only, favours its use as a model to study general features and response characteristics of airway epithelia in general. All epithelial cells are able to launch an immune response as characterized by the expression of antimicrobial peptide genes. Infection induces a complex change in the expression profile of these epithelial cells. Outstanding are a priming of the immune system and the launch of a survival program, presumably to counteract infection induced apoptotic signals, which comprises the concurrent expression of known longevity genes such as dFoxo, and dThor. In regions of the airway epithelium with strong immune reactions, a complex remodelling of the airways can be observed, which is characterized by metaplasia and presumably also by hyperplasia of the affected epithelial cells. At the transcriptional level, this reorganization of the airway epithelium is mirrored by a recapitulation of genetic programs that are characteristic for early phases of airway development. Taken together, the response characteristics of the fly’s airway epithelium towards infections discloses features that are known from inflammatory diseases of the human lung, thus opening the opportunity to study fundamental aspects of these diseases in the fly. Keywords:infection, Erwinia c., third instar larva, airway epithelium, two-colour microarray
2009-05-11 | GSE11444 | GEO
Project description:Larval host gene expression study in Drosophila post parasitic wasp-infection
| PRJNA133987 | ENA
Project description:ra14-05_multipass - effect of n nutrition of arabidopsis response to erwinia infection - Effect of N nutrition of Arabidopsis response to Erwinia infection - Comparison of Arabidopsis responses to Erwinia infection in relation to nitrogen nutrition (sufficient vs limited).
2017-04-12 | GSE97582 | GEO
Project description:Drosophila Eye Development - Larval Stage
| PRJNA84699 | ENA
Project description:The innate immune response of insects relies on several humoral and cellular mechanisms that require the activation of circulating proteases in the hemolymph to be functional. Here, we analyzed the gelatinase and caseinase activities of Drosophila larval hemolymph under normal and pathogenic conditions (bacterial lipopolysaccharides or endoparasitoid Leptopilina boulardi) using in gel zymography. Gelatinase activity was more intense than caseinase activity and qualitative and quantitative variations were observed between D. melanogaster strains and Drosophila species. Mass spectrometry identified a large number of serine proteases in gel bands equivalent to the major gelatinase and caseinase bands and of these, the most abundant and redundant were Tequila and members of the Jonah and Trypsin protease families. However, hemolymph from Tequila null mutant larvae showed no obvious changes in zymographic bands. Nor did we observe any significant changes in hemolymph gelatinases activity 24 h after injection of bacterial lipopolysaccharides or after oviposition by endoparasitoid wasps. These data confirmed that many serine proteases are present in Drosophila larval hemolymph but those with gelatinase and caseinase activity may not change drastically during the immune response.
2024-04-29 | PXD045527 | Pride
Project description:DamID data for SUUR binding in 12-14h Embryo, larval salivary glands and larval brain
| PRJNA148339 | ENA