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Geneship analysis of porcine femoral arteries after minimally invasive repair by different means of closure

ABSTRACT: Geneship analysis of porcine femoral arteries after minimally invasive repair by different means of closure

PROVIDER: PRJNA1091596 | ENA |

REPOSITORIES: ENA

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Geneship analysis of porcine femoral arteries after minimally invasive repair by different means of closure

Project description:After observing the surgical wounds on the 28th postoperative day, the experimental animals were put to death. Vascular tissues from the surgical site were taken and subjected to gene microarray analysis. This was used to examine the effect of TAG repair in the experimental group and Exoseal repair in the control group.

2024-03-30 | GSE262367 | GEO

Microarray analysis of femoral artery injury in mice

Project description:To determine the differential expression of genes at sites of vascular injury in mice Four male, 5-6 mo old SMA-GFP mice (numbered 57, 60, 61, and 63) were subjected to fine wire femoral artery injury. The left femoral artery of each mouse was injured, and the contralateral artery was used as an uninjured control. The mice recovered from the injury procedure for 14 days, at which time they were sacrificed. The femoral arteries were removed and the adventitial side was extensively cleaned. The arteries were carefully opened longitudinally and immediately imaged for GFP. GFP-negative regions of the arteries, representing sites of vascular injury were microdissected, quick-frozen on dry ice, and stored in liquid nitrogen. Uninjured arteries were isolated and analyzed similarly; the entire uninjured artery was frozen. Total RNA was prepared. A 100-ng portion of each sample was subjected to linear amplification and hybridized to Affymetrix mouse gene 1.0ST.

2012-09-10 | E-GEOD-40637 | biostudies-arrayexpress

Human femoral atheroma exhibit inflammation-resolving myeloid and lymphoid bias compared with carotid atheroma

Project description:Pathologic and clinical investigations suggest that femoral artery plaques are less inflammatory than other vascular beds, including carotid arteries. However, limited data exist regarding comparative immune landscapes and inflammatory polarization that may underlie such differences in femoral versus carotid plaque.

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Project description:Amphiuma means

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Gene expression differences between the brachial and femoral artery of the Rapacz HC swine

Project description:The age groups were used to investigate how gene expression differences between the brachial and the femoral artery effect the heterogeneous atherosclerotic disease initiation and progression. Total RNA was isolated from brachial and femoral arteries of four one week, six one year, and five two year old Rapacz HC swine.

2011-12-31 | E-GEOD-21043 | biostudies-arrayexpress

Differentially regulated miRNAs following murine femoral artery injury

Project description:We performed wire-induced injuries in 12 week old C57BL/6J mice. Dilation of the femoral artery was performed by inserting a straight spring wire (0.38 mm diameter) for 10 mm towards the iliac artery, as described previously (Sedding D, Daniel JM, Muhl L, Hersemeyer K, Brunsch H, Kemkes-Matthes B, Braun-Dullaeus RC, Tillmanns H, Weimer T, Preissner KT, Kanse SM. The g534e polymorphism of the gene encoding the factor vii-activating protease is associated with cardiovascular risk due to increased neointima formation. The Journal of experimental medicine. 2006;203:2801-2807). Mice were sacrificed and perfused with phosphate buffered saline via the left ventricle. Femoral arteries were excised at 10 and 21 days after injury, snap-frozen and miRNA was isolated by phenol-chloroform extraction following the purelink

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DNA methylation analysis of human atherosclerotic plaques in comparison to healthy mammary arteries

Project description:We report differential DNA methylation results from human femoral atherosclerotic plaques compared to healthy mammary arteries. 5-methylcytosine containing DNA fragments are captured by methylcytosine binding domain - based MethylCapture assay. Captured fragments are sequenced using Illumina paired-end approach and mapped to human genome (hg19). Results. Hypomethylation of chromosomal DNA predominates in atherosclerotic plaques. Chr14q32.2 was identified for the first time as an extensively hypomethylated area in atherosclerosis with highly induced expression of miR127, -136, -410, -431, and -432

2014-12-01 | GSE53575 | GEO

Genome-wide Methylation Profiles of femoral bones

Project description:Genome-wide DNA methylation profiling of femoral bones obtained from young and old individuals. The Illumina Infinium HumanMethylation EPIC BeadChip was used to obtain DNA methylation profiles across approximately 850K CpGs from human femoral bones.

2020-02-26 | GSE138307 | GEO

Expression data from human femoral artery atherosclerotic lesions

Project description:Paired samples from human femoral artery lesions were obtained during intravascular surgery exploiting Silverhawk device Microarrays were used to identify genes differentially regulated in human femoral artery atherosclerotic and corresponding restenotic plaques

2014-12-01 | GSE53274 | GEO

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