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Defective flow space limits the scaling up of turbulence bioreactors for platelet generation

ABSTRACT: Defective flow space limits the scaling up of turbulence bioreactors for platelet generation

PROVIDER: PRJNA1091907 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28457578.fastq.gz	Fastqsanger.gz
	SRR28457579.fastq.gz	Fastqsanger.gz
	SRR28457580.fastq.gz	Fastqsanger.gz
	SRR28457581.fastq.gz	Fastqsanger.gz
	SRR28457582.fastq.gz	Fastqsanger.gz

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Defective flow space limits the scaling up of turbulence bioreactors for platelet generation

Project description:To complement donor-dependent platelet supplies, we previously developed an ex vivo manufacturing system using iPSC-derived expandable megakaryocytes, imMKCLs, and a turbulent flow bioreactor, VerMES, to generate iPSC-derived platelet products (iPSC-PLTs). However, the tank size of VerMES was limited to 10 L (VerMES10). Here we examined the feasibility of scaling up to 50 L (VerMES50) with reciprocal motion by two impellers. Under optimized turbulence parameters corresponding to VerMES10, VerMES50 elicited iPSC-PLTs with intact in vivo hemostatic function but with less production efficiency. This insufficiency was caused by increased defective turbulent flow space. A computer simulation proposed that designing VerMES50 with three impellers or a new bioreactor with a modified rotating impeller and unique structure reduces this space. These findings indicate that large-scale PLT manufacturing from cultured imMKCLs requires optimization of the tank structure in addition to optimal turbulent energy and shear stress.

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